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Coordination environment of Cu(II) ions bound to N-terminal peptide fragments of angiogenin protein

Artikel i vetenskaplig tidskrift
Författare A. Magrì
A. Munzone
M. Peana
S. Medici
M. A. Zoroddu
Örjan Hansson
C. Satriano
E. Rizzarelli
D. La Mendola
Publicerad i International Journal of Molecular Sciences
Volym 17
Nummer/häfte 8
ISSN 1422-0067
Publiceringsår 2016
Publicerad vid Institutionen för kemi och molekylärbiologi
Språk en
Länkar dx.doi.org/10.3390/ijms17081240
Ämnesord Actin, Angiogenesis, Confocal microscopy, Copper, Neuroblastoma cells, NMR, Peptidomimetic, Recombinant protein, Stability constants
Ämneskategorier Biokemi och molekylärbiologi, Cellbiologi

Sammanfattning

Angiogenin (Ang) is a potent angiogenic factor, strongly overexpressed in patients affected by different types of cancers. The specific Ang cellular receptors have not been identified, but it is known that Ang–actin interaction induces changes both in the cell cytoskeleton and in the extracellular matrix. Most in vitro studies use the recombinant form (r-Ang) instead of the form that is normally present in vivo (“wild-type”, wt-Ang). The first residue of r-Ang is a methionine, with a free amino group, whereas wt-Ang has a glutamic acid, whose amino group spontaneously cyclizes in the pyro-glutamate form. The Ang biological activity is influenced by copper ions. To elucidate the role of such a free amino group on the protein–copper binding, we scrutinized the copper(II) complexes with the peptide fragments Ang(1–17) and AcAng(1–17), which encompass the sequence 1–17 of angiogenin (QDNSRYTHFLTQHYDAK-NH2), with free amino and acetylated N-terminus, respectively. Potentiometric, ultraviolet-visible (UV-vis), nuclear magnetic resonance (NMR) and circular dichroism (CD) studies demonstrate that the two peptides show a different metal coordination environment. Confocal microscopy imaging of neuroblastoma cells with the actin staining supports the spectroscopic results, with the finding of different responses in the cytoskeleton organization upon the interaction, in the presence or not of copper ions, with the free amino and the acetylated N-terminus peptides. © 2016 by the authors; licensee MDPI, Basel, Switzerland.

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