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Expression of inflammation/pain-related genes in the dorsal root ganglion following disc puncture in rats

Artikel i vetenskaplig tidskrift
Författare Yuki Fujioka
Anders Ståhlberg
M. Ochi
Kjell Olmarker
Publicerad i Journal of Orthopaedic Surgery
Volym 24
Nummer/häfte 1
Sidor 106-112
ISSN 1022-5536
Publiceringsår 2016
Publicerad vid Institutionen för biomedicin, avdelningen för patologi
Institutionen för biomedicin
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 106-112
Språk en
Ämnesord ganglia, spinal, gene expression profiling, low back pain, nucleus-pulposus, nerve root, receptor, pain, cells, Orthopedics, Surgery
Ämneskategorier Ortopedi

Sammanfattning

Purpose. To determine the expression of inflammation- and pain-related genes at days 1 and 3. in the dorsal root ganglion (DRG) of rats with or without disc puncture, using real-time quantitative polymerase chain reaction (RT-qPCR) with the TaqMan low-density array (TLDA). Methods. 53 female Sprague-Dawley rats were used. The left facet joint between L4 and L5 was removed, and the DRG and intervertebral disc between the vertebrae were exposed. The L4-5 intervertebral disc was punctured using a 0.4-mm diameter injection needle (disc puncture group) or left unpunctured (sham group). After one or 3 days, the 53 DRGs were harvested, frozen, and assessed for expression of inflammation-related genes. Total RNA was isolated from the DRGs. Expression of 119 genes related to inflammation and pain in the DRG after disc puncture were analysed using RT-qPCR with the TLDA. Results. Of the 95 inflammation-related genes, 78 genes were reliably detected. Two genes were significantly up-regulated: cysteinyl leukotriene receptor 1 (CYSLTR1) at day 3 and interleukin 2 receptor gamma (IL2RG) at day 1, and one gene was significantly down-regulated: phospholipase C beta 3 (PLCB3) at day 1. Of the 24 pa in-related genes, 18 genes were reliably detected. Two genes were significantly up-regulated: nitric oxide synthase 1 (NOS1) at days 1 and 3 and 5-HT2A receptor (HTR2A) at day 1. Conclusion. Disc puncture may elicit changes in the expression of a variety of genes. Gene expression profiling is a useful tool for detecting new potential pharmaceutical targets for spinal pain syndromes.

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