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Malignant pheochromocytomas/paragangliomas harbor mutations in transport and cell adhesion genes.

Artikel i vetenskaplig tidskrift
Författare Annica Wilzén
Anna Rehammar
Andreas Muth
Ola Nilsson
Tajana Tesan Tomic
Bo Wängberg
Erik Kristiansson
Frida Abel
Publicerad i International journal of cancer
Volym 138
Nummer/häfte 9
Sidor 2201-11
ISSN 1097-0215
Publiceringsår 2016
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk genetik och klinisk genetik
Institutionen för kliniska vetenskaper, Avdelningen för kirurgi
Institutionen för matematiska vetenskaper, matematisk statistik
Sahlgrenska Cancer Center
Institutionen för biomedicin, avdelningen för patologi
Sidor 2201-11
Språk en
Länkar dx.doi.org/10.1002/ijc.29957
Ämnesord neuroendocrine tumor;massively parallel sequencing;HRAS
Ämneskategorier Kirurgi

Sammanfattning

One out of ten patients with pheochromocytoma (PCC) and paraganglioma (PGL) develop malignant disease. Today there are no reliable pathological methods to predict malignancy at the time of diagnosis. Tumors harboring mutations in the succinate dehydrogenase subunit B (SDHB) gene often metastasize but the sequential genetic events resulting in malignant progression are not fully understood. The aim of this study was to identify somatic mutations that contribute to the malignant transformation of PCC/PGL. We performed pair-wise (tumor-normal) whole-exome sequencing to analyze the somatic mutational landscape in five malignant and four benign primary PCC/sympathetic PGL (sPGL), including two biological replicates from each specimen. In total, 225 unique somatic mutations were identified in 215 genes, with an average mutation rate of 0.54 mutations/megabase. Malignant tumors had a significantly higher number of mutations compared to benign tumors (p < 0.001). Three novel genes were identified as recurrently mutated; MYCN, MYO5B and VCL, and mutations in these genes were exclusively found in malignant sPGL tumors. Mutations in the MYO5B gene could be verified in two publicly available data sets. A gene ontology analysis of mutated genes showed enrichment of cellular functions related to cytoskeletal protein binding, myosin complex and motor activity, many of which had functions in Rab and Rac/Rho GTPase pathways. In conclusion, we have identified recurrent mutations in genes related to intracellular transport and cell adhesion, and we have confirmed MYO5B to be recurrently mutated in PCC/PGL cases with malignant potential. Our study suggests that deregulated Rab and Rac/Rho pathways may be important in PCC/PGL tumorigenesis.

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