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Solid phase extraction and metabolic profiling of exudates from living copepods

Artikel i vetenskaplig tidskrift
Författare Erik Selander
J. Heuschele
Göran M. Nylund
G. Pohnert
Henrik Pavia
O. Bjaerke
L. A. Pender-Healy
Peter Tiselius
T. Kiorboe
Publicerad i Peerj
Volym 4
ISSN 2167-8359
Publiceringsår 2016
Publicerad vid Institutionen för marina vetenskaper
Institutionen för biologi och miljövetenskap, Kristineberg
Språk en
Länkar dx.doi.org/10.7717/peerj.1529
Ämnesord Copepod exudate, Metabolomics, Infochemicals, Temora longicornis, Exometabolome, planktonic copepods, centropages-typicus, swimming behavior, chemical, ecology, cues, diatom, mate, phytoplankton, longicornis, ecosystems
Ämneskategorier Marin ekologi

Sammanfattning

Copepods are ubiquitous in aquatic habitats. They exude bioactive compounds that mediate mate finding or induce defensive traits in prey organisms. However, little is known about the chemical nature of the copepod exometabolome that contributes to the chemical landscape in pelagic habitats. Here we describe the development of a closed loop solid phase extraction setup that allows for extraction of exuded metabolites from live copepods. We captured exudates from male and female Temora longicornis and analyzed the content with high resolution LC-MS. Chemometric methods revealed 87 compounds that constitute a specific chemical pattern either qualitatively or quantitatively indicating copepod presence. The majority of the compounds were present in both female and male exudates, but nine compounds were mainly or exclusively present in female exudates and hence potential pheromone candidates. Copepodamide G, known to induce defensive responses in phytoplankton, was among the ten compounds of highest relative abundance in both male and female extracts. The presence of copepodamide G shows that the method can be used to capture and analyze chemical signals from living source organisms. We conclude that solid phase extraction in combination with metabolic profiling of exudates is a useful tool to develop our understanding of the chemical interplay between pelagic organisms.

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