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Flocked nasal swab versus nasopharyngeal aspirate in adult emergency room patients: similar multiplex PCR respiratory pathogen results and patient discomfort

Artikel i vetenskaplig tidskrift
Författare K. B. Hansen
Johan Westin
Lars-Magnus Andersson
Magnus Lindh
A. Widell
A. C. Nilsson
Publicerad i Infectious Diseases
Volym 48
Nummer/häfte 3
Sidor 246-250
ISSN 2374-4235
Publiceringsår 2016
Publicerad vid Institutionen för biomedicin, avdelningen för infektionssjukdomar
Sidor 246-250
Språk en
Länkar dx.doi.org/10.3109/23744235.2015.10...
Ämnesord Flocked nasal swabs, nasopharyngeal aspirates, respiratory infections, multiplex PCR, discomfort, polymerase-chain-reaction, nose-throat swabs, sampling methods, rapid, diagnosis, viruses, influenza, children, collection, symptoms, Infectious Diseases
Ämneskategorier Infektionsmedicin

Sammanfattning

Fifty adult emergency room patients with symptoms of respiratory tract infections or acute onset of extreme fatigue were sampled by both nasopharyngeal aspirate (NPA) and flocked nasal swab (fNS). Respiratory agents were detected by a qualitative influenza PCR and an 18-valent multiplex PCR in 20 of 29 patients with a clinical diagnosis of respiratory tract infection, and in 3 of 21 without such a diagnosis. PCR detected influenza A and B in NPA samples from 11 patients and in fNS samples from 10 patients. Little or no discomfort was perceived by 60% of the patients when sampled by NPA and by 66% when sampled by fNS. We conclude that NPA and fNS were equally sensitive for detection of respiratory agents by multiplex PCR, and the two sampling methods did not differ significantly regarding discomfort perceived by patients (p=0.171, Wilcoxon signed rank test). Hence less invasive sampling by fNS might be preferable in certain settings and situations.

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