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Feeding transgenic plants that express a tolerogenic fusion protein effectively protects against arthritis.

Artikel i vetenskaplig tidskrift
Författare Charlotta Hansson
Karin Schön
Irina Kalbina
Åke Strid
Sören Andersson
Maria Bokarewa
Nils Y Lycke
Publicerad i Plant biotechnology journal
Volym 14
Sidor 1106-1115
ISSN 1467-7652
Publiceringsår 2016
Publicerad vid Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning
Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi
Sidor 1106-1115
Språk en
Länkar dx.doi.org/10.1111/pbi.12479
Ämnesord vaccine
Ämneskategorier Medicinsk bioteknologi

Sammanfattning

Although much explored, oral tolerance for treatment of autoimmune diseases still awaits the establishment of novel and effective vectors. We investigated whether the tolerogenic CTA1(R7K)-COL-DD fusion protein can be expressed in edible plants, to induce oral tolerance and protect against arthritis. The fusion protein was recombinantly expressed in Arabidopsis thaliana plants, which were fed to H-2(q) -restricted DBA/1 mice to assess the preventive effect on collagen-induced arthritis (CIA). The treatment resulted in fewer mice exhibiting disease and arthritis scores were significantly reduced. Immune suppression was evident in treated mice, and serum biomarkers for inflammation as well as anticollagen IgG responses were reduced. In spleen and draining lymph nodes, CD4(+) T-cell responses were reduced. Concomitant with a reduced effector T-cell activity with lower IFNγ, IL-13 and IL-17A production, we observed an increase in IL-10 production to recall antigen stimulation in vitro, suggesting reduced Th1, Th2 and Th17 activity subsequent to up-regulated IL-10 and regulatory T-cell (Treg) functions. This study shows that edible plants expressing a tolerogen were effective at stimulating CD4 T-cell tolerance and in protecting against CIA disease. Our study conveys optimism as to the potential of using edible plants for oral treatment of rheumatoid arthritis.

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