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Targeted release and fractionation reveal glucuronylated and sulphated N- and O-glycans in larvae of dipteran insects

Artikel i vetenskaplig tidskrift
Författare S. Kurz
K. Aoki
Chunsheng S. Jin
Niclas G. Karlsson
M. Tiemeyer
I. B. H. Wilson
K. Paschinger
Publicerad i Journal of Proteomics
Volym 126
Sidor 172-188
ISSN 1874-3919
Publiceringsår 2015
Publicerad vid Institutionen för biomedicin
Sidor 172-188
Språk en
Länkar dx.doi.org/10.1016/j.jprot.2015.05....
Ämnesord Glycomics, Insects, Mass spectrometric, Glycans, Oligosaccharides, HPLC, ASPARAGINE-LINKED OLIGOSACCHARIDES, DROSOPHILA-MELANOGASTER EMBRYO, CELL-MEMBRANE GLYCOPROTEINS, T-ANTIGEN UNIT, HEPARAN-SULFATE, SUBSTRATE-SPECIFICITY, H-1-NMR SPECTROSCOPY, STRUCTURAL-ANALYSIS, ACIDIC, GLYCOLIPIDS, CALLIPHORA-VICINA, Biochemical Research Methods, ATES OF AMERICA, V104, P15882, NNIS RD, 1988, JOURNAL OF NEUROCHEMISTRY, V51, P1490, OFFYN P, 1963, BIOCHIMICA ET BIOPHYSICA ACTA, V70, P107
Ämneskategorier Medicinsk bioteknologi

Sammanfattning

Mosquitoes are important vectors of parasitic and viral diseases with Anopheles gambiae transmitting malaria and Aedes aegypti spreading yellow and Dengue fevers. Using two different approaches (solid-phase extraction and reversed-phase or hydrophilic interaction HPLC fractionation followed by MALDI-TOF MS or permethylation followed by NSI-MS), we examined the N-glycans of both A. gambiae and A. aegypti larvae and demonstrate the presence of a range of paucimannosidic glycans as well as bi- and tri-antennary glycans, some of which are modified with fucose or with sulphate or glucuronic acid residues; the latter anionic modifications were also found on N-glycans of larvae from another dipteran species (Drosophila melanogaster). The sulphate groups are attached primarily to core alpha-mannose residues (especially the alpha 1,6-linked mannose), whereas the glucuronic add residues are linked to non-reducing beta 1,3-galactose. Also, O-glycans were found to possess glucuronic acid and sulphate as well as phosphoethanolamine modifications. The presence of sulphated and glucuronylated N-glycans is a novel feature in dipteran glycomes; these structures have the potential to act as additional anionic glycan ligands involved in parasite interactions with the vector host. (C) 2015 Elsevier B.V. All rights reserved.

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