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Metabolomic and proteomic responses of Staphylococcus aureus to prolonged cold stress

Artikel i vetenskaplig tidskrift
Författare M. M. Alreshidi
R. H. Dunstan
M. M. Macdonald
N. D. Smith
Johan Gottfries
T. K. Roberts
Publicerad i Journal of Proteomics
Volym 121
Sidor 44-55
ISSN 1874-3919
Publiceringsår 2015
Publicerad vid Institutionen för kemi och molekylärbiologi
Sidor 44-55
Språk en
Länkar dx.doi.org/10.1016/j.jprot.2015.03....
Ämnesord Metabolomics, Proteomics, Phenomics, Citric acid, Ribosomal proteins, Cold stress, SMALL-COLONY VARIANTS, METHICILLIN-RESISTANT, MOONLIGHTING PROTEINS, INFECTIONS, PHYSIOLOGY, EVOLUTION, VIRULENCE, STRAIN, Biochemical Research Methods, JONGE BLM, 1992, JOURNAL OF BIOLOGICAL CHEMISTRY, V267, P11248
Ämneskategorier Mikrobiologi

Sammanfattning

The high pathogenicity of Staphylococcus aureus is thought to be due to its extraordinary capacity to rapidly adapt to changes in environmental conditions. This study was carried out to investigate whether the cytoplasmic profiles of metabolites and proteins of Staphylococcus aureus were altered in response to prolonged exposure to cold stress. Metabolic profiling and proteomics were used to characterise alterations in cytoplasmic proteins and metabolites in cells from the mid-exponential phase of growth under ideal conditions at 37 degrees C and compared with equivalent cells exposed to prolonged cold stress for 2 weeks at 4 degrees C. Principle component analysis (PCA) of the metabolomic and proteomic data indicated that, at the mid-exponential phase of growth, prolonged cold stress conditions generated cells with different metabolite and protein profiles compared with those grown at 37 degrees C. Nine ribosomal proteins and citric acid were substantially elevated in the cytoplasmic fractions from the cells adapted to cold-stress but most amino acids showed a reduction in their concentration in cold-stressed samples. The data provided strong evidence supporting the hypothesis that specific changes in metabolic homeostasis and protein composition were critical to the adaptive processes required for survival under cold stress. Work in our laboratory has shown that prolonged exposure of Staphylococcus aureus to cold stress can result in the formation of small colony variants (SCVs) associated with significant alterations in the cell wall composition [8]. Further studies revealed that Staphylococcus aureus altered cell size and cell wall thickness in response to exposure to cold temperatures, alterations in pH and exposure to antibiotics [10]. The current study has utilised the prolonged exposure to cold stress as a model system to explore changes in the proteome and associated metabolic homeostasis following environmental challenges. The study provides an improved understanding of how Staphylococcus aureus adapts to the changing environment whilst in transition between human hosts. The results indicated an unexpected production of 9 ribosomal proteins and citric acid in response to cold stress suggesting specific survival roles for these proteins and citric acid as an adaptation mechanism for empowering survival under these conditions. Crown Copyright (C) 2015 Published by Elsevier B.V. All rights reserved.

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