Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Novel rat models to study… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Novel rat models to study primary genital herpes simplex virus-2 infection

Artikel i vetenskaplig tidskrift
Författare Karin Önnheim
Maria Ekblad
Staffan Görander
Stefan Lange
Eva Jennische
Tomas Bergström
S. Wildt
Jan-Åke Liljeqvist
Publicerad i Archives of Virology
Volym 160
Nummer/häfte 5
Sidor 1153-1161
ISSN 0304-8608
Publiceringsår 2015
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Institutionen för biomedicin, avdelningen för infektionssjukdomar
Sidor 1153-1161
Språk en
Länkar dx.doi.org/10.1007/s00705-015-2365-...
Ämnesord GUINEA-PIGS, RECURRENT DISEASE, HSV-2 INFECTION, GLYCOPROTEIN G, TYPE-2, VACCINE, MICE, IMMUNITY, ANTIGEN, MOUSE, Virology
Ämneskategorier Virologi

Sammanfattning

In this study we describe that six rat models (SD, WIST, LEW, BN, F344 and DA) are susceptible to intravaginal herpes simplex virus-2 (HSV-2) infection after pre-treatment with progesterone. At a virus dose of 5 x 10(6) PFU of HSV-2, all rat models were infected presenting anti-HSV-2 antibodies, infectious virus in vaginal washes, and HSV-2 DNA genome copies in lumbosacral dorsal root ganglia and the spinal cord. Most of the LEW, BN, F344, and DA rats succumbed in systemic progressive symptoms at day 8-14 post infection, but presented no or mild genital inflammation while SD and WIST rats were mostly infected asymptomatically. Infected SD rats did not reactivate HSV-2 spontaneously or after cortisone treatment. In an HSV-2 virus dose reduction study, F344 rats were shown to be most susceptible. We also investigated whether an attenuated HSV-1 strain (KOS321) given intravaginally, could protect from a subsequent HSV-2 infection. All LEW, BN, and F344 rats survived a primary HSV-1 infection and no neuronal infection was established. In BN and F344 rats, anti-HSV-1 antibodies were readily detected while LEW rats were seronegative. In contrast to na < ve LEW, BN, and F344 rats where only 3 of 18 animals survived 5 x 10(6) PFU of HSV-2, 23 of 25 previously HSV-1 infected rats survived a challenge with HSV-2. The described models provide a new approach to investigate protective effects of anti-viral microbicides and vaccine candidates, as well as to study asymptomatic primary genital HSV-2 infection.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?