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Small changes in environmental parameters lead to alterations in antibiotic resistance, cell morphology and membrane fatty acid composition in Staphylococcus lugdunensis

Artikel i vetenskaplig tidskrift
Författare M. J. Crompton
R. H. Dunstan
M. M. Macdonald
Johan Gottfries
C. Von Eiff
T. K. Roberts
Publicerad i PLoS ONE
Volym 9
Nummer/häfte 4
ISSN 1932-6203
Förlag Public Library of Science
Publiceringsår 2014
Publicerad vid Institutionen för kemi och molekylärbiologi
Språk en
Länkar dx.doi.org/10.1371/journal.pone.009...
Ämnesord antibiotic agent, fatty acid, gentamicin, sodium chloride, antiinfective agent, adaptation, antibiotic resistance, antibiotic sensitivity, article, bacterial colonization, bacterial growth, bacterial membrane, bacterial survival, cell size, colony formation, controlled study, environmental stress, growth curve, growth rate, lipid composition, liquid culture, microbial morphology, minimum inhibitory concentration, nonhuman, osmotic pressure, pH, scanning electron microscopy, skin surface, Staphylococcus lugdunensis, temperature sensitivity, wound, cytology, drug effects, human, metabolism, microbiology, physiology, Staphylococcal Infections, temperature, Anti-Bacterial Agents, Drug Resistance, Microbial, Fatty Acids, Gentamicins, Humans, Hydrogen-Ion Concentration
Ämneskategorier Mikrobiologi


Staphylococcus lugdunensis has emerged as a major cause of community-acquired and nosocomial infections. This bacterium can rapidly adapt to changing environmental conditions to survive and capitalize on opportunities to colonize and infect through wound surfaces. It was proposed that S. lugdunensis would have underlying alterations in metabolic homeostasis to provide the necessary levels of adaptive protection. The aims of this project were to examine the impacts of subtle variations in environmental conditions on growth characteristics, cell size and membrane fatty acid composition in S. lugdunensis. Liquid broth cultures of S. lugdunensis were grown under varying combinations of pH (6-8), temperature (35-39°C) and osmotic pressure (0-5% sodium chloride w/w) to reflect potential ranges of conditions encountered during transition from skin surfaces to invasion of wound sites. The cells were harvested at the mid-exponential phase of growth and assessed for antibiotic minimal inhibitory concentration (MIC), generation time, formation of small colony variants, cell size (by scanning electron microscopy) and membrane fatty acid composition. Stress regimes with elevated NaCl concentrations resulted in significantly higher antibiotic resistance (MIC) and three of the combinations with 5% NaCl had increased generation times (P<0.05). It was found that all ten experimental growth regimes, including the control and centroid cultures, yielded significantly different profiles of plasma membrane fatty acid composition (P<0.0001). Alterations in cell size (P<0.01) were also observed under the range of conditions with the most substantial reduction occurring when cells were grown at 39°C, pH 8 (514±52 nm, mean ± Standard Deviation) compared with cells grown under control conditions at 37°C with pH 7 (702±76 nm, P<0.01). It was concluded that S. lugdunensis responded to slight changes in environmental conditions by altering plasma membrane fatty acid composition, growth rates and morphology to achieve optimal adaptations for survival in changing environments. © 2014 Crompton et al.

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