Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Separation and characteri… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Separation and characterization of hematosides with different sialic acids and ceramides from rat small intestine. Different composition of epithelial cells versus non-epithelial tissue and of duodenum versus jejunum-ileum.

Artikel i vetenskaplig tidskrift
Författare Jonas Ångström
Michael Breimer
K E Falk
I Griph
Gunnar C. Hansson
K A Karlsson
H Leffler
Publicerad i Journal of biochemistry
Volym 90
Nummer/häfte 4
Sidor 909-21
ISSN 0021-924X
Publiceringsår 1981
Publicerad vid Medicinska institutionen
Sidor 909-21
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Acetylation, Animals, Ceramides, analysis, Chromatography, Thin Layer, Epithelium, analysis, G(M3) Ganglioside, analysis, isolation & purification, Gangliosides, isolation & purification, Intestine, Small, analysis, Magnetic Resonance Spectroscopy, Male, Mass Spectrometry, Rats, Sialic Acids, analysis
Ämneskategorier Klinisk medicin

Sammanfattning

The hematosides (sialyl-lactosylceramides) of rat small intestine were separated as their acetylated derivatives. The isolated fractions were characterized by mass spectrometry and degradative methods, and the two major fractions also by NMR spectroscopy. From these results hematosides with different sialic acid and ceramide type could be assigned to thin-layer chromatographic bands. This allowed a structural interpretation of the chromatographic patterns observed for different parts of the small intestine. Thus, epithelial cells of ileum contained only hematoside with N-glycoloylneuraminic acid. Duodenum lacked this compound and instead the epithelial cells contained hematoside with N-acetylneuraminic acid. In non-epithelial tissue or both duodenum and jejunum-ileum the major hematoside had N-acetyl-neuraminic acid. The hematosides of epithelial cells had ceramide containing 18 : 0 trihydroxy base combined with 16, 20, 22, 24 : 0, and 24 : 1 hydroxy fatty acids (major part) or non-hydroxy fatty acids. In the non-epithelial hematosides the ceramide consisted of 18 : 1 dihydroxy base combined with 16, 18, 20, 22, 24 : 0, and 24 : 1 non-hydroxy fatty acids.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?