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Using imaging ToF-SIMS data to determine the cell wall thickness of fibers in wood

Paper i proceeding
Författare Lorenz Gerber
V. M. Hoang
L. Tran
H. A. T. Kiet
Per Malmberg
Jörg Hanrieder
Andrew G Ewing
Publicerad i Surface and Interface Analysis: 19th International Conference on Secondary Ion Mass Spectrometry (SIMS)
Volym 46
Sidor 225-228
ISSN 0142-2421
Publiceringsår 2014
Publicerad vid Institutionen för kemi och molekylärbiologi
Sidor 225-228
Språk en
Länkar dx.doi.org/10.1002/sia.5661
Ämnesord ToF-SIMS, image analysis, wood analysis
Ämneskategorier Fysikalisk kemi

Sammanfattning

Here, we demonstrate the use of time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging data for estimation of cell wall thickness in wood samples. Current research in forest biotechnology focuses on transgenic trees with wood properties tailored to specific applications. Appropriate analytical methods to characterize the very heterogeneous wood material are constantly being developed and improved. Chemical imaging of wood by ToF-SIMS represents an interesting tool for this purpose with many applications. In addition to wood chemistry, the impact of specific genetic modifications on wood anatomy needs to be assessed. Cell wall thickness is an important anatomical parameter that among others is used for assessing biomass accumulation. We developed a strategy to estimate cell wall thickness from ToF-SIMS images and implemented it in the open source programming language R'. In brief, random lines are projected over the black and white mask of a ToF-SIMS image, and length values of all line sections that cut across a cell wall are collected. After enough iteration, the shortest values of the obtained count distribution represent the crossing sections normal to the cell walls, hence cell wall thickness. Compared with conventional light microscopy image analysis, TOF-SIMS data offers many advantages such as submicron resolution and additional spectral information for automated annotation of distinct anatomical features. This work underlines the importance of SIMS imaging for studies of wood chemistry and anatomy and provides a new approach to obtain an important wood anatomical parameter from ToF-SIMS data.

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