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Development of intestinal ion-transporting mechanisms during smoltification and seawater acclimation in Atlantic salmon Salmo salar

Artikel i vetenskaplig tidskrift
Författare Henrik Sundh
T. O. Nilsen
Jenny Lindström
Linda Hasselberg Frank
S. O. Stefansson
S. D. McCormick
Kristina Sundell
Publicerad i Journal of Fish Biology
Volym 85
Nummer/häfte 4
Sidor 1227-1252
ISSN 0022-1112
Publiceringsår 2014
Publicerad vid Institutionen för biologi och miljövetenskap
Sidor 1227-1252
Språk en
Länkar dx.doi.org/10.1111/jfb.12531
Ämnesord cystic fibrosis transmembrane conductance regulator, fluid absorption, Na+/K+-ATPase, Na+/K+/2Cl(-), PARR-SMOLT TRANSFORMATION, K-CL COTRANSPORTER, CATION-CHLORIDE-COTRANSPORTERS, TROUT ONCORHYNCHUS-MYKISS, RAINBOW-TROUT, FRESH-WATER, MARINE TELEOST, NA+/K+-ATPASE, BROWN TROUT, OSMOREGULATORY PHYSIOLOGY, Fisheries, Marine & Freshwater Biology
Ämneskategorier Miljövetenskap

Sammanfattning

This study investigated the expression of ion transporters involved in intestinal fluid absorption and presents evidence for developmental changes in abundance and tissue distribution of these transporters during smoltification and seawater (SW) acclimation of Atlantic salmon Salmo salar. Emphasis was placed on Na+, K+-ATPase (NKA) and Na+, K+, Cl- co-transporter (NKCC) isoforms, at both transcriptional and protein levels, together with transcription of chloride channel genes. The nka alpha 1c was the dominant isoform at the transcript level in both proximal and distal intestines; also, it was the most abundant isoform expressed in the basolateral membrane of enterocytes in the proximal intestine. This isoform was also abundantly expressed in the distal intestine in the lower part of the mucosal folds. The protein expression of intestinal Nka alpha 1c increased during smoltification. Immunostaining was localized to the basal membrane of the enterocytes in freshwater (FW) fish, and re-distributed to a lateral position after SW entry. Two other Nka isoforms, alpha 1a and alpha 1b, were expressed in the intestine but were not regulated to the same extent during smoltification and subsequent SW transfer. Their localization in the intestinal wall indicates a house-keeping function in excitatory tissues. The absorptive form of the NKCC-like isoform (sub-apically located NKCC2 and/or Na+, Cl(-)co-transporter) increased during smoltification and further after SW transfer. The cellular distribution changed from a diffuse expression in the sub-apical regions during smoltification to clustering of the transporters closer to the apical membrane after entry to SW. Furthermore, transcript abundance indicates that the mechanisms necessary for exit of chloride ions across the basolateral membrane and into the lateral intercellular space are present in the form of one or more of three different chloride channels: cystic fibrosis transmembrane conductance regulator I and II and chloride channel 3.

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