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Membrane Protein Profiling of Human Colon Reveals Distinct Regional Differences

Artikel i vetenskaplig tidskrift
Författare Sjoerd van der Post
Gunnar C. Hansson
Publicerad i Molecular & Cellular Proteomics
Volym 13
Nummer/häfte 9
Sidor 2277-2287
ISSN 1535-9476
Publiceringsår 2014
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 2277-2287
Språk en
Länkar dx.doi.org/10.1074/mcp.M114.040204
Ämnesord INTESTINAL EPITHELIAL-CELLS, O-GLYCOSYLATION, MUC2 MUCIN, EXPRESSION, TRANSPORTER, ANOCTAMINS, COLITIS, DISEASE, CANCER
Ämneskategorier Biokemi

Sammanfattning

The colonic epithelium is a highly dynamic system important for the regulation of ion and water homeostasis via absorption and secretion and for the maintenance of a protective barrier between the outer milieu and the inside of the body. These processes are known to gradually change along the length of the colon, although a complete characterization at the protein level is lacking. We therefore analyzed the membrane proteome of isolated human (n = 4) colonic epithelial cells from biopsies obtained via routine colonoscopy for four segments along the large intestine: ascending, transverse, descending, and sigmoid colon. Label-free quantitative proteomic analyses using high-resolution mass spectrometry were performed on enriched membrane proteins. The results showed a stable level for the majority of membrane proteins but a distinct decrease in proteins associated with bacterial sensing, cation transport, and O-glycosylation in the proximal to distal regions. In contrast, proteins involved in microbial defense and anion transport showed an opposing gradient and increased toward the distal end. The gradient of ion-transporter proteins could be directly related to previously observed ion transport activities. All individual glycosyltransferases required for the O-glycosylation of the major colonic mucin MUC2 were observed and correlated with the known glycosylation variation along the colon axis. This is the first comprehensive quantitative dataset of membrane protein abundance along the human colon and will add to the knowledge of the physiological function of the different regions of the colonic mucosa. Mass spectrometry data have been deposited to the ProteomeXchange with the identifier PXD000987.

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