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Calcium imaging in the zebrafish.

Kapitel i bok
Författare Petronella Kettunen
Publicerad i Calcium Signaling. Advances in Experimental Medicine and Biology, Vol. 740, Ed. Shahidul Islam
Volym 740
Sidor 1039-71
ISBN 978-94-007-2887-5
ISSN 0065-2598
Förlag Springer
Publiceringsår 2012
Publicerad vid Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi
Sidor 1039-71
Språk en
Länkar dx.doi.org/10.1007/978-94-007-2888-...
Ämnesord Animals, Calcium, analysis, metabolism, Muscles, chemistry, metabolism, Myocardium, chemistry, metabolism, Neurons, chemistry, metabolism, Smell, Spinal Cord, chemistry, metabolism, Zebrafish, embryology, metabolism
Ämneskategorier Cellbiologi, Neurovetenskaper

Sammanfattning

The zebrafish (Danio rerio) has emerged as a new model system during the last three decades. The fact that the zebrafish larva is transparent enables sophisticated in vivo imaging. While being the vertebrate, the reduced complexity of its nervous system and small size make it possible to follow large-scale activity in the whole brain. Its genome is sequenced and many genetic and molecular tools have been developed that simplify the study of gene function. Since the mid 1990s, the embryonic development and neuronal function of the larval, and later, adult zebrafish have been studied using calcium imaging methods. The choice of calcium indicator depends on the desired number of cells to study and cell accessibility. Dextran indicators have been used to label cells in the developing embryo from dye injection into the one-cell stage. Dextrans have also been useful for retrograde labeling of spinal cord neurons and cells in the olfactory system. Acetoxymethyl (AM) esters permit labeling of larger areas of tissue such as the tectum, a region responsible for visual processing. Genetically encoded calcium indicators have been expressed in various tissues by the use of cell-specific promoters. These studies have contributed greatly to our understanding of basic biological principles during development and adulthood, and of the function of disease-related genes in a vertebrate system.

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