Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Allosteric regulation of … - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells

Artikel i vetenskaplig tidskrift
Författare Anna-Karin Gustavsson
David D. van Niekerk
Caroline B. Adiels
Bob Kooi
Mattias Goksör
Jacky L. Snoep
Publicerad i The FEBS Journal
Volym 281
Nummer/häfte 12
Sidor 2784-2793
ISSN 1742-464X
Publiceringsår 2014
Publicerad vid Institutionen för fysik (GU)
Sidor 2784-2793
Språk en
Länkar dx.doi.org/10.1111/febs.12820
Ämnesord cell heterogeneity, glycolysis, limit cycle oscillation, mathematical model, microfluidic chamber
Ämneskategorier Fysik, Optik, Biokemi, Bioinformatik och systembiologi

Sammanfattning

Oscillations are widely distributed in nature and synchronization of oscillators has been described at the cellular level (e.g. heart cells) and at the population level (e.g. fireflies). Yeast glycolysis is the best known oscillatory system, although it has been studied almost exclusively at the population level (i.e. limited to observations of average behaviour in synchronized cultures). We studied individual yeast cells that were positioned with optical tweezers in a microfluidic chamber to determine the precise conditions for autonomous glycolytic oscillations. Hopf bifurcation points were determined experimentally in individual cells as a function of glucose and cyanide concentrations. The experiments were analyzed in a detailed mathematical model and could be interpreted in terms of an oscillatory manifold in a three-dimensional state-space; crossing the boundaries of the manifold coincides with the onset of oscillations and positioning along the longitudinal axis of the volume sets the period. The oscillatory manifold could be approximated by allosteric control values of phosphofructokinase for ATP and AMP.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?