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Assessment of tissue viability following electroosmotic push-pull perfusion from organotypic hippocampal slice cultures

Artikel i vetenskaplig tidskrift
Författare A. E. Rupert
Y. Ou
Mats Sandberg
S. G. Weber
Publicerad i ACS Chemical Neuroscience
Volym 4
Nummer/häfte 5
Sidor 849-857
ISSN 1948-7193
Publiceringsår 2013
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 849-857
Språk en
Länkar dx.doi.org/10.1021/cn4000814
Ämnesord electroosmosis, injury, Organotypic hippocampal cultures, push-pull perfusion, sampling, propidium iodide, animal experiment, article, brain perfusion, capillary, cell death, controlled study, electric field, electric potential, extracellular space, geometry, hippocampus, nonhuman, priority journal, rat, Animals, Electric Stimulation, Organ Culture Techniques, Perfusion, Rats, Rats, Sprague-Dawley, Tissue Survival
Ämneskategorier Biokemi, Neurokemi

Sammanfattning

We have developed a novel sampling technique that allows both introduction and removal of fluid from the extracellular space of living tissue. This method is based on the fluidics of push-pull perfusion but flow is driven by electroosmosis. We have applied this method to organotypic hippocampal cultures. A source capillary is inserted into the tissue and a collection capillary is in contact with the tissue surface through a thin layer of fluid. A voltage is applied across the proximal ends of source and collection capillary. In the applied field, fluid will move from source, into the tissue, and then be collected. In this process, damage to cells may occur. To understand better what sampling conditions influence damage most, we tested various sampling geometries and applied voltages, quantifying damage 16-24 h later using propidium iodide as a cell death marker. We found that damage correlates with both voltage drop and power dissipated in the tissue, but that voltage drop is a better indicator of damage when comparing models in which capillary arrangement and length are different. © 2013 American Chemical Society.

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