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Genotyping of Pseudomonas aeruginosa isolates from lung transplant recipients and aquatic environment-detected in-hospital transmission

Artikel i vetenskaplig tidskrift
Författare Ewa Johansson
Christina Welinder-Olsson
Marita Gilljam
Publicerad i Apmis
Volym 122
Nummer/häfte 2
Sidor 85-91
ISSN 0903-4641
Publiceringsår 2014
Publicerad vid Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition
Institutionen för biomedicin, avdelningen för infektionssjukdomar
Sidor 85-91
Språk en
Länkar dx.doi.org/10.1111/apm.12243
Ämnesord Pseudomonas aeruginosa, lung transplant, transmission, surveillance, genotyping, CYSTIC-FIBROSIS PATIENTS, INTENSIVE-CARE-UNIT, FIELD, GEL-ELECTROPHORESIS, INFECTION, STRAINS, CLONE, MORTALITY, OUTBREAK
Ämneskategorier Klinisk medicin

Sammanfattning

Lung infection with Pseudomonas aeruginosa is common in lung transplant recipients and may lead to severe complications. Bacteriological surveillance aims to detect transmission of microbes between hospital environment and patients. We sought to determine whether genotyping of P. aeruginosa isolates could improve identifications of pathways of infection. From 2004 to 2009, we performed genotyping with multiple-locus variable number of tandem repeats analysis (MLVA) and pulsed-field gel electrophoresis (PFGE) of P. aeruginosa isolates cultured from lung transplant recipients at Sahlgrenska University Hospital, Gothenburg. During a small outbreak in 2008, cultivation and genotyping of isolates from sink and drains samples from the hospital ward were performed. Pseudomona aeruginosa from 11/18 patients were genotyped to unique strains. The remaining seven patients were carriers of a P. aeruginosa strain of cluster A genotype. Pseudomona aeruginosa was isolated in 4/8 water samples, typed by MLVA also as cluster A genotype and confirmed by PFGE to be similar or identical to the isolates from four transplanted patients. In conclusion, genotyping of isolates revealed a clonal relationship between patient and water isolates, indicating in-hospital transmission of P. aeruginosa. We suggest genotyping with MLVA for rapid routine surveillance, with the PFGE method used for extended, confirmatory analyses.

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