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Direct interaction between cholera toxin and dendritic cells is required for oral adjuvant activity

Artikel i vetenskaplig tidskrift
Författare Tobias Gustafsson
Yeu-Jiann Hua
M. W. Dahlgren
Megan Livingston
B. Johansson-Lindbom
Ulf Yrlid
Publicerad i European Journal of Immunology
Volym 43
Nummer/häfte 7
Sidor 1779-1788
ISSN 0014-2980
Publiceringsår 2013
Publicerad vid Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi
Sidor 1779-1788
Språk en
Länkar dx.doi.org/10.1002/eji.201242867
Ämnesord Cholera toxin, DC, IgA, Mucosal immunity, Oral adjuvants, HEAT-LABILE ENTEROTOXIN, IN-VIVO, T-CELL, BYSTANDER ACTIVATION, IMMUNE-RESPONSES, PEYERS-PATCHES, IMMUNIZATION, MIGRATION, FLAGELLIN, RECEPTOR, ATES OF AMERICA, V109, P2072
Ämneskategorier Klinisk medicin

Sammanfattning

Cholera toxin (CT) binds to GM1-ganglioside receptors present on all nucleated cells. Despite this, it is a very potent mucosal adjuvant that has a dramatic impact on immune cells, as well as nerve and epithelial cells, causing diarrhea. This fact has hampered our understanding of whether the adjuvanticity of CT is direct or indirect, as cells that bind CT may or may not be involved in its adjuvant function. The mucosal barrier is maintained by tight junctions between epithelial cells but dendritic cells (DCs) can protrude luminal dendrites. Here we investigated which cells are involved in the immune augmenting effect of CT. We explored oral immunizations with ovalbumin (OVA) and CT in bone marrow chimeric mice deficient in GM1-ganglioside in defined cellular subsets. We found that chimeric mice lacking GM1 in nonhematopoietic cells, including epithelial cells, mounted an unaltered intestinal IgA response. In contrast, chimeric mice lacking GM1-expressing hematopoietic cells in general, or specifically GM1-expressing conventional DCs (cDCs), largely failed to elicit anti-OVA adaptive immune responses. Therefore, the adjuvanticity of CT does not require epithelial activation, but is directly dependent on the binding of CT to gut cDCs via GM1-ganglioside. These results could have important implications for the generation of novel oral adjuvants.

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