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Universal method for protein bioconjugation with nanocellulose scaffolds for increased cell adhesion

Artikel i vetenskaplig tidskrift
Författare Volodymyr Kuzmenko
Sanna Sämfors
Daniel Hägg
Paul Gatenholm
Publicerad i Materials Science & Engineering C-Materials for Biological Applications
Volym 33
Nummer/häfte 8
Sidor 4599-4607
ISSN 0928-4931
Publiceringsår 2013
Publicerad vid
Sidor 4599-4607
Språk en
Ämnesord Nanocellulose, Protein bioconjugation, Cell adhesion, Cell morphology, POLYSACCHARIDE CONJUGATE VACCINES, BACTERIAL CELLULOSE, 1-CYANO-4-DIMETHYLAMINOPYRIDINIUM TETRAFLUOROBORATE, SOLUBLE, POLYSACCHARIDES, IMMUNOLOGICAL REAGENTS, MECHANICAL-PROPERTIES, EXTRACELLULAR-MATRIX, ACETOBACTER-XYLINUM, POTENTIAL SCAFFOLD, ACTIVATION
Ämneskategorier Kemi

Sammanfattning

Bacterial nanocellulose (BNC) is an emerging biomaterial since it is biocompatible, integrates well with host tissue and can be biosynthesized in desired architecture. However, being a hydrogel, it exhibits low affinity for cell attachment, which is crucial for the cellular fate process. To increase cell attachment, the surface of BNC scaffolds was modified with two proteins, fibronectin and collagen type I, using an effective bioconjugation method applying 1-cyano-4-dimethylaminopyridinium (CDAP) tetrafluoroborate as the intermediate catalytic agent. The effect of CDAP treatment on cell adhesion to the BNC surface is shown for human umbilical vein endothelial cells and the mouse mesenchymal stem cell line C3H10T1/2. In both cases, the surface modification increased the number of cells attached to the surfaces. In addition, the morphology of the cells indicated more healthy and viable cells. CDAP activation of bacterial nanocellulose is shown to be a convenient method to conjugate extracellular proteins to the scaffold surfaces. CDAP treatment can be performed in a short period of time in an aqueous environment under heterogeneous and mild conditions preserving the nanofibrillar network of cellulose.

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