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Studies of mucus in mouse stomach, small intestine, and colon. III. Gastrointestinal Muc5ac and Muc2 mucin O-glycan patterns reveal a regiospecific distribution

Artikel i vetenskaplig tidskrift
Författare Jessica Holmén Larsson
Kristina A Thomsson
Ana María Rodríguez-Piñeiro
Hasse Karlsson
Gunnar C. Hansson
Publicerad i American Journal of Physiology-Gastrointestinal and Liver Physiology
Volym 305
Nummer/häfte 5
Sidor G357-G363
ISSN 0193-1857
Publiceringsår 2013
Publicerad vid Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor G357-G363
Språk en
Länkar dx.doi.org/10.1152/ajpgi.00048.2013
Ämnesord glycomics, Mucin, Muc2, Muc5ac, mass spectrometry, oligosaccharide, glycan, BLOOD-GROUP-A, LINKED OLIGOSACCHARIDES, NIPPOSTRONGYLUS-BRASILIENSIS, HELICOBACTER-PYLORI, GUT MICROBIOTA, GLYCOSYLATION, MICE, GLYCOSYLTRANSFERASE, DIVERSITY, BACTERIA, ATES OF AMERICA, Animals, Chromatography, Liquid, Colon, metabolism, Electrophoresis, Polyacrylamide Gel, Female, Fucose, metabolism, Gastric Mucosa, metabolism, Glycosylation, Intestinal Mucosa, metabolism, Intestine, Small, metabolism, Male, Mass Spectrometry, Mice, Mice, Inbred C57BL, Mucin 5AC, metabolism, Mucin-2, metabolism, Mucus, metabolism, N-Acetylneuraminic Acid, metabolism, Proteomics, methods, Sulfates, metabolism
Ämneskategorier Klinisk medicin, Hälsovetenskaper

Sammanfattning

The mouse intestinal mucus is mainly made up by the gel-forming Muc2 mucin and the stomach surface mucus Muc5ac, both extensively O-glycosylated. The oligosaccharide diversity provides a vast library of potential recognition sites for both commensal and pathogenic organisms. The mucin glycans are thus likely very important for the selection and maintenance of a stable intestinal flora. Here we have explored the O-glycan patterns of the mouse gastrointestinal tract mucins. The mucins from the mucus of the distal and proximal colon, ileum, jejunum, duodenum, and stomach of conventionally raised wild-type (C57BL/6) mice were separated by composite gel electrophoresis. The O-linked glycans were released by reductive elimination and structurally characterized by liquid chromatography-mass spectrometry. The mucins glycans were mostly core 2 type [Gal beta 1-3(GlcNAc beta 1-6)GalNAcol], but also core 1 (Gal beta 1-3GalNAcol). In the stomach about half of the Muc5ac mucin O-glycans were neutral and many monosulfated, but with a low grade of sialylation and fucosylation. Mouse ileum, jejunum, and duodenum had similar glycan patterns dominated by sialylated and sulfated core 2 glycans, but few fucosylated. Colon was on the other hand dominated by highly charged fucosylated glycans. The distal colon is different from the proximal colon because different biosynthetic pathways are utilized, although sialylated and sulfated glycans were highly abundant in both parts. The sulfation was higher in the distal colon, whereas sialic acid was more common in the proximal colon. Many fucosylated glycans were found in both the proximal and distal colon. Thus the mucin O-glycans vary along the mouse gastrointestinal tract.

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