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Freeze-Drying as Sample Preparation for Micellar Electrokinetic Capillary Chromatography-Electrochemical Separations of Neurochemicals in Drosophila Brains

Artikel i vetenskaplig tidskrift
Författare E. C. Berglund
N. J. Kuklinski
E. Karagunduz
Kubra Ucar
Jörg Hanrieder
Andrew G Ewing
Publicerad i Analytical Chemistry
Volym 85
Nummer/häfte 5
Sidor 2841-2846
ISSN 0003-2700
Publiceringsår 2013
Publicerad vid
Sidor 2841-2846
Språk en
Ämneskategorier Analytisk kemi

Sammanfattning

Micellar electrokinetic capillary chromatography with electrochemical detection has been used to quantify biogenic amines in freeze-dried brains of Drosophila melanogaster. Freeze-drying samples offers a way to preserve the biological sample while making dissection of these tiny samples easier and faster. Fly samples were extracted in cold acetone and dried in a rotary evaporator. Extraction and drying times were optimized in order to avoid contamination by red pigment from the fly eyes and still have intact brain structures. Single freeze-dried fly brain samples were found to produce representative electropherograms as a single hand-dissected brain sample. With utilization of the faster dissection time that freeze-drying affords, the number of brains in a fixed homogenate volume can be increased to concentrate the sample. Thus, concentrated brain samples containing five or fifteen preserved brains were analyzed for their neurotransmitter content, and four analytes; N-acetyloctopamine, N-acetylserotonin, N-acetyltyramine, and N-acetyldopamine were found to correspond well with previously reported values.

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