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Lactobacilli from human gastrointestinal mucosa are strong stimulators of IL-12 production.

Artikel i vetenskaplig tidskrift
Författare Christina Hessle
Lars Åke Hanson
Agnes E Wold
Publicerad i Clinical and experimental immunology
Volym 116
Nummer/häfte 2
Sidor 276-82
ISSN 0009-9104
Publiceringsår 1999
Publicerad vid Institutionen för laboratoriemedicin, Avdelningen för klinisk immunologi
Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi
Sidor 276-82
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Cells, Cultured, Escherichia coli, physiology, Gastric Mucosa, microbiology, Humans, Interleukin-10, biosynthesis, Interleukin-12, biosynthesis, Intestinal Mucosa, microbiology, Lactobacillus, physiology, Leukocytes, Mononuclear, metabolism, Monocytes, metabolism
Ämneskategorier Mikrobiologi inom det medicinska området, Immunologi inom det medicinska området

Sammanfattning

Interaction of macrophages with bacteria is a stimulus for production of cytokines such as IL-10 and IL-12. IL-12 stimulates T cell and natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) production. IL-10 opposes the T cell-stimulating action of IL-12, decreases the release of proinflammatory cytokines from macrophages, and stimulates B cells. We have studied the capacity of human intestinal isolates from the three Lactobacillus species dominating on the human gastrointestinal mucosa, L. plantarum, L. rhamnosus and L. paracasei ssp. paracasei, to induce production of IL-10 and IL-12 from human blood mononuclear cells, or monocytes. Whole killed lactobacilli were potent stimulators of IL-12 over a wide range of bacterial concentrations. Lactobacillus paracasei gave the highest levels of IL-12 (1.5 ng/ml in response to 5 x 106 bacteria/ml), roughly 10 times more than obtained by stimulation with L. rhamnosus or L. plantarum. Escherichia coli induced on average < 50 pg/ml of IL-12 regardless of the bacterial concentration used. The secretion of free p40 subunit IL-12 followed the same pattern as the secretion of p70 (bioactive IL-12) with regard to the efficiency of different bacteria as stimulators. Escherichia coli was the most efficient trigger of IL-10 production, inducing 0.5 ng/ml IL-10 after stimulation with 5 x 106 bacteria/ml. Lactobacillus rhamnosus induced the highest levels of IL-10 among the lactobacilli (0.5 ng/ml) compared with 0.1 ng/ml evoked by L. plantarum or L. paracasei, but 10 times more bacteria were required for optimal stimulation than with E. coli. When neutralizing anti-IL-10 antibodies were added to the cultures, the IL-12-inducing capacity of L. rhamnosus was increased markedly, while that of E. coli remained low. The results show that mucosa-associated lactobacilli can be potent stimulators of IL-12, and thus potentially of cell-mediated immunity, if they pass over the gut epithelial barrier and interact with cells of the gut immune system.

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