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Gram-positive bacteria are potent inducers of monocytic interleukin-12 (IL-12) while gram-negative bacteria preferentially stimulate IL-10 production.

Artikel i vetenskaplig tidskrift
Författare Christina Hessle
Bengt A. Andersson
Agnes E Wold
Publicerad i Infection and immunity
Volym 68
Nummer/häfte 6
Sidor 3581-6
ISSN 0019-9567
Publiceringsår 2000
Publicerad vid Institutionen för laboratoriemedicin, Avdelningen för klinisk immunologi
Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi
Sidor 3581-6
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Aerobiosis, Anaerobiosis, Gram-Negative Bacteria, immunology, Gram-Positive Bacteria, immunology, Histocompatibility Antigens Class II, immunology, Humans, Interferon-gamma, biosynthesis, Interleukin-10, antagonists & inhibitors, biosynthesis, immunology, Interleukin-12, biosynthesis, Lipopolysaccharides, biosynthesis, Monocytes, immunology, Th1 Cells, immunology, Tumor Necrosis Factor-alpha, biosynthesis
Ämneskategorier Immunologi inom det medicinska området, Mikrobiologi inom det medicinska området

Sammanfattning

Interleukin-10 (IL-10) and IL-12 are two cytokines secreted by monocytes/macrophages in response to bacterial products which have largely opposite effects on the immune system. IL-12 activates cytotoxicity and gamma interferon (IFN-gamma) secretion by T cells and NK cells, whereas IL-10 inhibits these functions. In the present study, the capacities of gram-positive and gram-negative bacteria to induce IL-10 and IL-12 were compared. Monocytes from blood donors were stimulated with UV-killed bacteria from each of seven gram-positive and seven gram-negative bacterial species representing both aerobic and anaerobic commensals and pathogens. Gram-positive bacteria induced much more IL-12 than did gram-negative bacteria (median, 3,500 versus 120 pg/ml at an optimal dose of 25 bacteria/cell; P < 0.001), whereas gram-negative bacteria preferentially stimulated secretion of IL-10 (650 versus 200 pg/ml; P < 0.001). Gram-positive species also induced stronger major histocompatibility complex class II-restricted IFN-gamma production in unfractionated blood mononuclear cells than did gram-negative species (12,000 versus 3,600 pg/ml; P < 0.001). The poor IL-12-inducing capacity of gram-negative bacteria was not remediated by addition of blocking anti-IL-10 antibodies to the cultures. No isolated bacterial component could be identified that mimicked the potent induction of IL-12 by whole gram-positive bacteria, whereas purified LPS induced IL-10. The results suggest that gram-positive bacteria induce a cytokine pattern that promotes Th1 effector functions.

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