Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Astrocytes negatively reg… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Astrocytes negatively regulate neurogenesis through the Jagged1-mediated notch pathway.

Artikel i vetenskaplig tidskrift
Författare Ulrika Wilhelmsson
Maryam Faiz
Yolanda de Pablo
Marika Sjöqvist
Daniel Andersson
Åsa Widestrand
Maja Potokar
Matjaž Stenovec
Peter L P Smith
Noriko Shinjyo
Tulen Pekny
Robert Zorec
Anders Ståhlberg
Marcela Pekna
Cecilia Sahlgren
Milos Pekny
Publicerad i Stem cells (Dayton, Ohio)
Volym 30
Nummer/häfte 10
Sidor 2320-9
ISSN 1549-4918
Publiceringsår 2012
Publicerad vid Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering
Institutionen för neurovetenskap och fysiologi, sektionen för farmakologi
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 2320-9
Språk en
Länkar dx.doi.org/10.1002/stem.1196
Ämnesord Astrocytes, Glial fibrillary acidic protein, Vimentin, Intermediate filaments, Neurogenesis
Ämneskategorier Medicinska grundvetenskaper, Neurobiologi

Sammanfattning

Adult neurogenesis is regulated by a number of cellular players within the neurogenic niche. Astrocytes participate actively in brain development, regulation of the mature central nervous system (CNS), and brain plasticity. They are important regulators of the local environment in adult neurogenic niches through the secretion of diffusible morphogenic factors, such as Wnts. Astrocytes control the neurogenic niche also through membrane-associated factors, however, the identity of these factors and the mechanisms involved are largely unknown. In this study, we sought to determine the mechanisms underlying our earlier finding of increased neuronal differentiation of neural progenitor cells when cocultured with astrocytes lacking glial fibrillary acidic protein (GFAP) and vimentin (GFAP(-/-) Vim(-/-) ). We used primary astrocyte and neurosphere cocultures to demonstrate that astrocytes inhibit neuronal differentiation through a cell-cell contact. GFAP(-/-) Vim(-/-) astrocytes showed reduced endocytosis of Notch ligand Jagged1, reduced Notch signaling, and increased neuronal differentiation of neurosphere cultures. This effect of GFAP(-/-) Vim(-/-) astrocytes was abrogated in the presence of immobilized Jagged1 in a manner dependent on the activity of γ-secretase. Finally, we used GFAP(-/-) Vim(-/-) mice to show that in the absence of GFAP and vimentin, hippocampal neurogenesis under basal conditions as well as after injury is increased. We conclude that astrocytes negatively regulate neurogenesis through the Notch pathway, and endocytosis of Notch ligand Jagged1 in astrocytes and Notch signaling from astrocytes to neural stem/progenitor cells depends on the intermediate filament proteins GFAP and vimentin.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?