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Human Embryonic Stem Cell-Derived Mesodermal Progenitors Display Substantially Increased Tissue Formation Compared to Human Mesenchymal Stem Cells Under Dynamic Culture Conditions in a Packed Bed/Column Bioreactor.

Artikel i vetenskaplig tidskrift
Författare Giuseppe Maria de Peppo
Martina Sladkova
Peter Sjövall
Anders Palmquist
Karim Oudina
Johan Hyllner
Peter Thomsen
Hervé Petite
Camilla Karlsson
Publicerad i Tissue engineering. Part A
Volym 19
Nummer/häfte 1-2
Sidor 175-87
ISSN 1937-335X
Publiceringsår 2013
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap
Sidor 175-87
Språk en
Länkar dx.doi.org/10.1089/ten.TEA.2011.041...
https://gup.ub.gu.se/file/121161
Ämneskategorier Klinisk medicin, Biomaterialvetenskap

Sammanfattning

Bone tissue engineering represents a promising strategy to obviate bone deficiencies, allowing the ex vivo construction of bone substitutes with unprecedented potential in the clinical practice. Considering that in the human body cells are constantly stimulated by chemical and mechanical stimuli, the use of bioreactor is emerging as an essential factor for providing the proper environment for the reproducible and large-scale production of the engineered substitutes. Human mesenchymal stem cells (hMSCs) are experimentally relevant cells but, regardless the encouraging results reported after culture under dynamic conditions in bioreactors, show important limitations for tissue engineering applications, especially considering their limited proliferative potential, loss of functionality following protracted expansion, and decline in cellular fitness associated with aging. On the other hand, we previously demonstrated that human embryonic stem cell-derived mesodermal progenitors (hES-MPs) hold great potential to provide a homogenous and unlimited source of cells for bone engineering applications. Based on prior scientific evidence using different types of stem cells, in the present study we hypothesized that dynamic culture of hES-MPs in a packed bed/column bioreactor had the potential to affect proliferation, expression of genes involved in osteogenic differentiation, and matrix mineralization, therefore resulting in increased bone-like tissue formation. The reported findings suggest that hES-MPs constitute a suitable alternative cell source to hMSCs and hold great potential for the construction of bone substitutes for tissue engineering applications in clinical settings.

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