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Intraluminal Polyethylene Glycol Stabilizes Tight Junctions and Improves Intestinal Preservation in the Rat

Artikel i vetenskaplig tidskrift
Författare Mihai Oltean
Meghnad Joshi
Eleonora Björkman
S Oltean
Anna Casselbrant
Gustaf Herlenius
Michael Olausson
Publicerad i American Journal of Transplantation
Volym 12
Nummer/häfte 8
Sidor 2044-2051
ISSN 1600-6135
Publiceringsår 2012
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för kirurgi
Institutionen för kliniska vetenskaper, Avdelningen för gastrokirurgisk forskning och utbildning
Sidor 2044-2051
Språk en
Länkar dx.doi.org/10.1111/j.1600-6143.2012...
Ämnesord Intestinal preservation, luminal preservation, polyethylene glycol, preservation injury, preservation, small-bowel preservation, cavitary 2-layer method, canine, small-intestine, luminal preservation, proteins, expression, injury, ischemia, claudins, barrier
Ämneskategorier Kirurgi

Sammanfattning

Rapidly progressing mucosal breakdown limits the intestinal preservation time below 10 h. Recent studies indicate that intraluminal solutions containing polyethylene glycol (PEG) alleviate preservation injury of intestines stored in UW-Viaspan. We investigated whether a low-sodium PEG solution is beneficial for intestines stored in histidine-tryptophane-ketoglutarate (HTK) preservation solution. Rat intestines used as control tissue (group 1) were perfused with HTK, groups 2 and 3 received either a customized PEG-3350 (group 2) or an electrolyte solution (group 3) intraluminally before cold storage. Tissue injury, brush-border maltase activity, zonula occludens-1 (ZO-1) and claudin-3 expression in the tight junctions (TJ) were analyzed after 8, 14 and 20 h. We measured epithelial resistance and permeability (Ussing chamber) after 8 and 14 h. Group 2 had superior morphology while maltase activity was similar in all groups. TJ proteins rapidly decreased and decolocalized in groups 1 3; these negative events were delayed in group 2, where colocalization persisted for about 14 h. Intestines in group 2 had higher epithelial resistance and lower permeability than the other groups. These results suggest that a customized PEG solution intraluminally reduces the intestinal preservation injury by improving several major epithelial characteristics without negatively affecting the brush-border enzymes or promoting edema.

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