Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Detection of human papill… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Detection of human papillomavirus oncoprotein E7 in liquid-based cytology

Artikel i vetenskaplig tidskrift
Författare Maria Lidqvist
Olle Nilsson
Jan Holmgren
Sebastian Hölters
Eva Röijer
Matthias Dürst
Christian Fermér
Publicerad i The Journal of general virology
Volym 93
Nummer/häfte 2
Sidor 356-363
ISSN 1465-2099
Publiceringsår 2012
Publicerad vid Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi
Sidor 356-363
Språk en
Länkar dx.doi.org/10.1099/vir.0.034884-0
Ämnesord Animals, Antibodies, Monoclonal, diagnostic use, Antibodies, Viral, diagnostic use, Blotting, Western, Early Detection of Cancer, methods, Epitope Mapping, Female, Humans, Immunohistochemistry, methods, Mice, Mice, Inbred BALB C, Papillomavirus E7 Proteins, analysis, Papillomavirus Infections, diagnosis, Sensitivity and Specificity
Ämneskategorier Medicinska grundvetenskaper, Immunologi inom det medicinska området

Sammanfattning

The selection and characterization of a set of mouse mAbs against high-risk human papillomavirus (HPV) E7 oncoprotein and the development of protocols for immunocytochemistry (ICC) are described here. A large number of antibodies raised towards HPV16 and 18 E7 were tested for high-risk specificity by ELISA using a panel of HPV E7 proteins. Antibodies detecting low-risk E7 were discarded, resulting in 38 high-risk HPV E7-specific antibodies. The corresponding epitopes were mapped using overlapping HPV E7 fragments displayed on phage particles. Functionality in ICC against formalin-fixed cervical cancer cell lines was demonstrated for ten mAbs; their high-risk specificity was confirmed by Western blot analysis and ICC on transiently transformed cells expressing high- or low-risk HPV E7. These mAbs were specific for one or several of the high-risk strains HPV16, 18, 31, 35 and 45. Specific E7 staining of liquid-based cytology (LBC) samples was demonstrated for seven mAbs and optimized protocols were established. The E716-41 and E718-79 mAbs demonstrated particularly strong and specific staining of cells stored in LBC fluid for at least 6 months. It is proposed that the high-risk HPV E7 staining protocols established in this study may have the potential to be included in a complementary test for the detection and identification of malignantly transformed cells, in for example atypical squamous cells of undetermined significance samples.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?