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Cross Validation of Liquid Chromatography-Mass Spectrometry and Lectin Array for Monitoring Glycosylation in Fed-Batch Glycoprotein Production

Artikel i vetenskaplig tidskrift
Författare Catherine A Hayes
R. Doohan
D. Kirkley
K. Leister
B. Harhen
A. V. Savage
Niclas G. Karlsson
Publicerad i Molecular Biotechnology
Volym 51
Nummer/häfte 3
Sidor 272-282
ISSN 1073-6085
Publiceringsår 2012
Publicerad vid Institutionen för biomedicin
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 272-282
Språk en
Länkar dx.doi.org/10.1007/s12033-011-9465-...
Ämnesord Glycosylation, High mannose, Acetylation, N-linked oligosaccharides, Lectin array, Mass spectrometry, hamster ovary cells, protein, microarray, glycomics, strategy, glycans, oligosaccharides, expression, antibodies, ammonia
Ämneskategorier Medicinsk bioteknologi

Sammanfattning

Glycosylation analysis of recombinant glycoproteins is of importance for the biopharmaceutical industry and the production of glycoprotein pharmaceuticals. A commercially available lectin array technology was evaluated for its ability to present a reproducible fingerprint of a recombinant CTLY4-IgG fusion glycoprotein expressed in large scale CHO-cell fermentation. The glycosylation prediction from the array was compared to traditional negative mode capillary LC-MS of released oligosaccharides. It was shown that both methods provide data that allow samples to be distinguished by their glycosylation pattern. This included information about sialylation, the presence of reducing terminal galactose beta 1-, terminal N-acetylglucosamine beta 1-, and antennary distribution. With both methods it was found that a general trend of increased sialylation was associated with an increase of the antenna and reduced amount of terminal galactose beta 1-, while N-acetylglucosamine beta 1- was less affected. LC-MS, but not the lectin array, provided valuable information about the sialic acid isoforms present, including N-acetylneuraminic acid, N-glycolylneuraminic acid and their O-acetylated versions. Detected small amounts of high-mannose structures by LC-MS correlated with the detection of the same epitope by the lectin array.

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