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Characterization of mRNA and microRNA in human mast cell-derived exosomes and their transfer to other mast cells and blood CD34 progenitor cells

Artikel i vetenskaplig tidskrift
Författare Karin Ekström
Hadi Valadi
Margareta Sjöstrand
Carina Malmhäll
Apostolos Bossios
Maria Eldh
Jan Lötvall
Publicerad i Journal of Extracellular Vesicles (JEV)
Volym 1
Sidor 18389
ISSN 2001-3078
Publiceringsår 2012
Publicerad vid Krefting Research Centre
Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap
Institutionen för medicin, avdelningen för invärtesmedicin
Sidor 18389
Språk en
Länkar dx.doi.org/10.3402/jev.v1i0.18389
Ämnesord exosomes, extracellular vesicles, human mast cells, RNA, esRNA
Ämneskategorier Medicinsk cellbiologi

Sammanfattning

Background: Exosomes are nanosized vesicles of endocytic origin that are released into the extracellular environment by many different cells. It has been shown that exosomes from various cellular origins contain a substantial amount of RNA (mainly mRNA and microRNA). More importantly, exosomes are capable of delivering their RNA content to target cells, which is a novel way of cell-to-cell communication. The aim of 20 this study was to evaluate whether exosomal shuttle RNA could play a role in the communication between human mast cells and between human mast cells and human CD34+progenitor cells. Methods: The mRNA and microRNA content of exosomes from a human mast cell line, HMC-1, was analysed by using microarray technology. Co-culture experiments followed by flow cytometry analysis and confocal microscopy as well as radioactive labeling experiments were performed to examine the uptake of 25 these exosomes and the shuttle of the RNA to other mast cells and CD34+ progenitor cells. Results: In this study, we show that human mast cells release RNA-containing exosomes, with the capacity to shuttle RNA between cells. Interestingly, by using microRNA microarray analysis, 116 microRNAs could be identified in the exosomes and 134 microRNAs in the donor mast cells. Furthermore, DNA microarray experiments revealed the presence of approximately 1800 mRNAs in the exosomes, which represent 15% of 30 the donor cell mRNA content. In addition, transfer experiments revealed that exosomes can shuttle RNA between human mast cells and to CD34+ hematopoietic progenitor cells. Conclusion: These findings suggest that exosomal shuttle RNA (esRNA) can play a role in the communication between cells, including mast cells and CD34+ progenitor cells, implying a role in cells maturation process. 35

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