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The stimulation of an osteogenic response by classical monocyte activation.

Artikel i vetenskaplig tidskrift
Författare Omar Omar
Cecilia Granéli
Karin Ekström
Camilla Karlsson
Anna Johansson
Jukka Lausmaa
Cecilia Larsson Wexell
Peter Thomsen
Publicerad i Biomaterials
Volym 32
Nummer/häfte 32
Sidor 8190–8204
ISSN 1878-5905
Publiceringsår 2011
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap
Sidor 8190–8204
Språk en
Länkar dx.doi.org/10.1016/j.biomaterials.2...
Ämnesord Monocytes, Inflammation, Mesenchymal stem cells, Cell signalling, Cytokines, Osseointegration
Ämneskategorier Biomaterialvetenskap, Klinisk medicin

Sammanfattning

The monocyte/macrophage system plays a central role in host defense, wound healing and immune regulation at biomaterial surfaces. Monocytes can be classically and alternatively activated, and can be stimulated differently in response to variations in biomaterial surface properties. In this study, human monocytes, cultured on polystyrene surfaces (Ps), were activated either classically, by lipopolysaccharide (LPS), or alternatively, by interleukin-4 (IL-4). Monocytes were also cultured on anodically oxidized (Ox) and machined (Ma) titanium surfaces, with and without LPS stimulation. Cells were cultured for 1 and 3 days and their conditioned media (CM) were collected. The osteogenic response of hMSCs to the monocyte CM was determined by analyzing the gene expression of key osteogenic markers. The CM from classically activated monocytes increased the hMSCs expression of runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP). Furthermore, CM from monocytes cultured on Ox surface resulted in a modest increase of the expression of bone morphogenetic protein-2 (BMP-2). LPS stimulation of the surface-seeded monocytes overwhelmed the effect of the surface properties and resulted in significant upregulation of BMP-2 and Runx2 for all samples. The results show that human monocytes, cultured on different surfaces and/or under different activation pathways, communicate pro-osteogenic signals to hMSCs. The signals involve regulation of autologous BMP-2 in the hMSCs. The classical activation results in profound and prolonged osteogenic effect compared to the effect of the investigated surface properties.

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