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Characterization of Carrageenan Extracted from Hypnea bryoides in Oman.

Artikel i vetenskaplig tidskrift
Författare Ahmed Ali Al-Alawi
Insaaf Mohammed Al-Marhubi
Mohammed Said Moosa Al-Belushi
Bassam Soussi
Publicerad i Marine biotechnology (New York, N.Y.)
Volym 10
Nummer/häfte 1007
Sidor 10126
ISSN 1436-2236
Publiceringsår 2011
Publicerad vid Institutionen för kliniska vetenskaper, Avdelningen för kirurgi
Sidor 10126
Språk en
Länkar dx.doi.org/10.1007/s10126-010-9350-...
Ämneskategorier Medicin och Hälsovetenskap

Sammanfattning

Carrageenophyte red seaweed from Oman, Hypnea bryoides, extracted using three different processes: an aqueous, a mild alkaline, and a more vigorous alkaline extraction was investigated. The resulting extract precipitated by alcohol was subject to chemical and rheological measurements. The total carbohydrate [ranged from 36.78 to 41.65 g/100 g], and ash [39.04 to 43.11 g/100 g] were the most abundant components in H. bryoides and contrary to the two, lipid content was found at a minimum [ranging from 2.95 to 3.38 g/100 g]. Alkali treatment with NaOH allowed complete conversion of kappa (κ) carrageenan form as detected by FTIR analysis. Total yield by alkali treatments gave higher yields (33%) compared with aqueous treatments (12%). However, subsequent aqueous treatment produced mixed carrageenan (μ and κ) with higher molecular weight compared with the alkali treatments which produced single carrageenan form (κ) with molecular weight of 4.1 × 10(5) Da. The effects of thermal history on gel-sol and sol-gel transition were investigated by differential scanning calorimeter (DSC) and rheology on a pure sample and 1.5% κ-carrageenan mixture added with 30 mM KCl. Transition temperatures from DSC and rheology showed comparable results and were in good agreement with those previously reported.

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