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Gram-positive and gram-negative bacteria induce different patterns of cytokine production in human mononuclear cells irrespective of taxonomic relatedness.

Artikel i vetenskaplig tidskrift
Författare Susann Skovbjerg
Anna Martner
Lars Hynsjö
Christina Hessle
Ingar Olsen
Floyd E Dewhirst
Wilhelm Tham
Agnes E Wold
Publicerad i Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
Volym 30
Nummer/häfte 1
Sidor 23-32
ISSN 1557-7465
Publiceringsår 2010
Publicerad vid Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin
Institutionen för biomedicin, avdelningen för infektionssjukdomar
Sidor 23-32
Språk en
Länkar dx.doi.org/10.1089/jir.2009.0033
Ämnesord Antigens, Bacterial, immunology, Cells, Cultured, Cytokines, metabolism, Enzyme-Linked Immunosorbent Assay, Gram-Negative Bacteria, immunology, Gram-Positive Bacteria, immunology, Humans, Immunity, Innate, Inflammation Mediators, metabolism, Leukocytes, Mononuclear, immunology, metabolism, microbiology, pathology, Lymphocyte Activation, Phagocytosis, immunology, Species Specificity, Ultraviolet Rays
Ämneskategorier Mikrobiologi inom det medicinska området, Bakteriologi, Immunologi inom det medicinska området

Sammanfattning

Upon bacterial stimulation, tissue macrophages produce a variety of cytokines that orchestrate the immune response that clears the infection. We have shown that Gram-positives induce higher levels of interleukin-12 (IL-12), interferon-gamma (IFN-gamma), and tumor necrosis factor (TNF) from human peripheral blood mononuclear cells (PBMCs) than do Gram-negatives, which instead induce more of IL-6, IL-8, and IL-10. Here, we study whether these patterns follows or crosses taxonomic borders. PBMCs from blood donors were incubated with UV-inactivated bacteria representing 37 species from five phyla. IL-12, TNF, IL-1beta, IL-6, IL-8, and IL-10 were measured in the supernatants after 24 h and IFN-gamma after 5 days. Irrespective of phylogenetic position, Gram-positive bacteria induced much more IL-12 (nine times more on average) and IFN-gamma (seven times), more TNF (three times), and slightly more IL-1beta (1.5 times) than did Gram-negatives, which instead induced more IL-6 (1.5 times), IL-8 (1.9 times), and IL-10 (3.3 times) than did Gram-positives. A notable exception was the Gram-positive Listeria monocytogenes, which induced very little IL-12, IFN-gamma, and TNF. The results confirm the fundamental difference in innate immune responses to Gram-positive and Gram-negative bacteria, which crosses taxonomic borders and probably reflects differences in cell wall structure.

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