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Genetics of extracellular matrix remodeling during organ growth using the Caenorhabditis elegans pharynx model.

Artikel i vetenskaplig tidskrift
Författare Gholamali Jafari
Jan Burghoorn
Takehiro Kawano
Manoj Mathew
Catarina Mörck
Claes Axäng
Michael Ailion
James H Thomas
Joseph G Culotti
Peter Swoboda
Marc Pilon
Publicerad i Genetics
Volym 186
Nummer/häfte 3
Sidor 969-82
ISSN 1943-2631
Publiceringsår 2010
Publicerad vid Institutionen för cell- och molekylärbiologi
Sidor 969-82
Språk en
Länkar dx.doi.org/10.1534/genetics.110.120...
Ämneskategorier Biologiska vetenskaper, Cell- och molekylärbiologi, Molekylärbiologi, Morfologi, Genetik, Utvecklingsbiologi, Neurobiologi

Sammanfattning

The organs of animal embryos are typically covered with an extracellular matrix (ECM) that must be carefully remodeled as these organs enlarge during post-embryonic growth; otherwise, their shape and functions may be compromised. We previously described the twisting of the Caenorhabditis elegans pharynx (here called the Twp phenotype) as a quantitative mutant phenotype that worsens as that organ enlarges during growth. Mutations previously known to cause pharyngeal twist affect membrane proteins with large extracellular domains (DIG-1 and SAX-7), as well as a C. elegans septin (UNC-61). Here we show that two novel alleles of the C. elegans papilin gene, mig-6(et4) and mig-6(sa580), can also cause the Twp phenotype. We also show that overexpression of the ADAMTS protease gene mig-17 can suppress the pharyngeal twist in mig-6 mutants and identify several alleles of other ECM-related genes that can cause or influence the Twp phenotype, including alleles of fibulin (fbl-1), perlecan (unc-52), collagens (cle-1, dpy-7), laminins (lam-1, lam-3), one ADAM protease (sup-17), and one ADAMTS protease (adt-1). The Twp phenotype in C. elegans is easily monitored using light microscopy, is quantitative via measurements of the torsion angle, and reveals that ECM components, metalloproteinases, and ECM attachment molecules are important for this organ to retain its correct shape during post-embryonic growth. The Twp phenotype is therefore a promising experimental system to study ECM remodeling and diseases.

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