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In vivo cytokine secretion and NF-kappaB activation around titanium and copper implants.

Artikel i vetenskaplig tidskrift
Författare Felicia Suska
Christina Gretzer
Marco Esposito
Lena Emanuelsson
Ann Wennerberg
Pentti Tengvall
Peter Thomsen
Publicerad i Biomaterials
Volym 26
Nummer/häfte 5
Sidor 519-27
ISSN 0142-9612
Publiceringsår 2005
Publicerad vid Institutionen för de kirurgiska disciplinerna, Avdelningen för biomaterialvetenskap
Sidor 519-27
Språk en
Länkar dx.doi.org/10.1016/j.biomaterials.2...
Ämnesord Animals, Cell Survival, Chemotaxis, Leukocyte, Coated Materials, Biocompatible, Copper, Exudates and Transudates, Female, Foreign-Body Reaction, etiology, metabolism, Implants, Experimental, Interleukin-10, secretion, Interleukin-6, secretion, Materials Testing, Monocytes, metabolism, secretion, NF-kappa B, metabolism, Neutrophils, metabolism, secretion, Rats, Rats, Sprague-Dawley, Titanium
Ämneskategorier Biomaterialvetenskap, Klinisk medicin

Sammanfattning

The early biological response at titanium (Ti), copper (Cu)-coated Ti and sham sites was evaluated in an in vivo rat model. Material surface chemical and topographical properties were characterized using Auger electron spectroscopy, energy dispersive X-ray spectroscopy and interferometry, respectively. The number of leukocytes, cell types and cell viability (release of lactate dehydrogenase) were determined in the implant-interface exudate. The contents of activated nuclear transcription factor NF-kappaB, interleukin-6 (IL-6) and interleukin-10 (IL-10) were determined by enzyme linked immunosorbent assay. An increase in the number of leukocytes, in particular, polymorphonuclear leukocytes, was observed between 12 and 48 h around Cu. A marked decrease of exudate cell viability was found around Cu after 48 h. The total amounts of activated NF-kappaB after 12 h was highest in Ti exudates whereas after 48 h the highest amount of NF-kappaB was detected around Cu. The levels of cytokine IL-6 were consistently high around Cu at both time periods. No differences in IL-10 contents were detected, irrespective of material/sham and time. The results show that materials with different toxicity grades (titanium with low and copper with high toxicity) exhibit early differences in the activation of NF-kappaB, extracellular expression and secretion of mediators, causing major differences in inflammatory cell accumulation and death in vivo.

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