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Intranasal immunization with a proteoliposome-derived cochleate containing recombinant gD protein confers protective immunity against genital herpes in mice.

Artikel i vetenskaplig tidskrift
Författare Judith Del Campo
Madelene Lindqvist
Maribel Cuello
Malin Bäckström
Osmir Cabrerra
Josefine Persson
Oliver Perez
Ali M Harandi
Publicerad i Vaccine
Volym 28
Nummer/häfte 5
Sidor 1193-200
ISSN 1873-2518
Publiceringsår 2010
Publicerad vid Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi
Core Facilities, Mammalian Protein Expression
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 1193-200
Språk en
Länkar dx.doi.org/10.1016/j.vaccine.2009.1...
Ämnesord Administration, Intranasal, Animals, Antibodies, Viral, immunology, Antigens, Bacterial, immunology, pharmacology, CD4-Positive T-Lymphocytes, immunology, Female, Herpes Genitalis, genetics, immunology, prevention & control, Herpesvirus 2, Human, immunology, Immunization, Immunoglobulin G, immunology, Interferon-gamma, immunology, Liposomes, Mice, Mice, Knockout, Myeloid Differentiation Factor 88, genetics, immunology, Neisseria meningitidis, Serogroup B, immunology, Recombinant Proteins, genetics, immunology, pharmacology, Viral Envelope Proteins, genetics, immunology, pharmacology
Ämneskategorier Mikrobiologi inom det medicinska området

Sammanfattning

The purpose of this study was to investigate the potential of intranasal (IN) immunization with Neisseria meningitides B proteoliposome (AFPL1) and AFPL1-derived cochleate (AFCo1), containing glycoprotein D (gD) of herpes simplex virus type 2 (HSV-2) for induction of protective immunity against genital herpes infection in mice. We could show that IN immunization with both AFPL1 and AFCo1 containing gD induced gD-specific IgG antibody and lymphoproliferative responses. However, IFN-gamma response could only be detected in CD4(+) splenic cells and genital lymph node cells of the AFCo1gD immunized mice upon recall antigen stimulation in vitro. Importantly, IN immunization with AFCo1gD could elicit a complete protection against an otherwise lethal vaginal challenge with HSV-2, while the AFPL1gD immunized mice were only partially protected. Further, we could show that the IFN-gamma response and protective immunity observed after IN immunization with AFCo1gD are mediated via the adaptor molecule myeloid differentiation factor 88. These data may have implications for the development of a mucosal vaccine against genital herpes.

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