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Genome-wide occupancy profile of mediator and the Srb8-11 module reveals interactions with coding regions.

Artikel i vetenskaplig tidskrift
Författare Xuefeng Zhu
Marianna Wirén
Indranil Sinha
Nina N Rasmussen
Tomas Linder
Steen Holmberg
Karl Ekwall
Claes M Gustafsson
Publicerad i Molecular cell
Volym 22
Nummer/häfte 2
Sidor 169-78
ISSN 1097-2765
Publiceringsår 2006
Publicerad vid
Sidor 169-78
Språk en
Ämnesord Alleles, Chromatin Immunoprecipitation, DNA, Fungal, DNA, Intergenic, Down-Regulation, Gene Expression Profiling, Genes, Fungal, Genome, Fungal, Nucleic Acid Amplification Techniques, Oligonucleotide Array Sequence Analysis, Open Reading Frames, Promoter Regions, Genetic, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae, genetics, metabolism, Saccharomyces cerevisiae Proteins, genetics, metabolism, Schizosaccharomyces, genetics, metabolism, Schizosaccharomyces pombe Proteins, genetics, metabolism, Transcription, Genetic
Ämneskategorier Kemi

Sammanfattning

Mediator exists in a free form containing the Med12, Med13, CDK8, and CycC subunits (the Srb8-11 module) and a smaller form, which lacks these four subunits and associates with RNA polymerase II (Pol II), forming a holoenzyme. We use chromatin immunoprecipitation (ChIP) and DNA microarrays to investigate genome-wide localization of Mediator and the Srb8-11 module in fission yeast. Mediator and the Srb8-11 module display similar binding patterns, and interactions with promoters and upstream activating sequences correlate with increased transcription activity. Unexpectedly, Mediator also interacts with the downstream coding region of many genes. These interactions display a negative bias for positions closer to the 5' ends of open reading frames (ORFs) and appear functionally important, because downregulation of transcription in a temperature-sensitive med17 mutant strain correlates with increased Mediator occupancy in the coding region. We propose that Mediator coordinates transcription initiation with transcriptional events in the coding region of eukaryotic genes.

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