Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Identification of tryptop… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Identification of tryptophan hydroxylase as an intestinal autoantigen.

Artikel i vetenskaplig tidskrift
Författare Olov Ekwall
H Hedstrand
L Grimelius
J Haavik
J Perheentupa
J Gustafsson
E Husebye
O Kämpe
F Rorsman
Publicerad i Lancet
Volym 352
Nummer/häfte 9124
Sidor 279-83
ISSN 0140-6736
Publiceringsår 1998
Publicerad vid
Sidor 279-83
Språk en
Ämnesord Adolescent, Adult, Autoantibodies, analysis, Autoantigens, immunology, Autoimmune Diseases, immunology, Blood Donors, Child, DNA, Complementary, Duodenum, immunology, Female, Finland, epidemiology, Genes, Recessive, Humans, Immunohistochemistry, Intestine, Small, immunology, Male, Middle Aged, Norway, epidemiology, Polyendocrinopathies, Autoimmune, classification, epidemiology, genetics, immunology, Sweden, epidemiology, Tryptophan Hydroxylase, immunology
Ämneskategorier Endokrinologi, Gastroenterologi

Sammanfattning

BACKGROUND: Autoimmune polyendocrine syndrome type 1 (APS1) is an autosomal recessive disorder with both endocrine and non-endocrine features. Periodic gastrointestinal dysfunction occurs in 25-30% of APS1 patients. We aimed to identify an intestinal autoantigen. METHODS: A human duodenal cDNA library was immunoscreened with serum samples from APS1 patients. A positive clone was identified and used for in-vitro transcription and translation, followed by immunoprecipitation with serum samples from 80 APS1 patients from Norway, Finland, and Sweden. Sections of normal and APS1-affected small intestine were immunostained with serum from APS1 patients and specific antibodies. An enzyme-inhibition assay was used to characterise the autoantibodies. FINDINGS: We isolated a cDNA clone coding for tryptophan hydroxylase. 48% (38/80) of APS1 patients had antibodies to tryptophan hydroxylase, whereas no reactivity to this antigen was detected in patients with other autoimmune diseases (n=372) or healthy blood donors (n=70). 89% (17/19) of APS1 patients with gastrointestinal dysfunction were positive for antibodies to tryptophan hydroxylase, compared with 34% (21/61) of patients with no gastrointestinal dysfunction (p<0.0001). Serum from antibody-positive APS1 patients specifically immunostained tryptophan-hydroxylase-containing enterochromaffin cells in normal duodenal mucosa. No serotonin-containing cells were seen in duodenal biopsy samples from APS1 patients. Serum from antibody-positive APS1 patients almost completely inhibited activity of tryptophan hydroxylase. INTERPRETATION: Tryptophan hydroxylase is an endogenous intestinal autoantigen in APS1, and there is an association between antibodies to the antigen and gastrointestinal dysfunction. Analysis of antibodies to tryptophan hydroxylase may be a valuable diagnostic tool to predict and monitor gastrointestinal dysfunction in APS1.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?