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Nanotube-vesicle networks with functionalized membranes and interiors

Artikel i vetenskaplig tidskrift
Författare M. Davidson
M. Karlsson
J. Sinclair
Kristin Sott
Owe Orwar
Publicerad i Journal of the American Chemical Society
Volym 125
Nummer/häfte 2
Sidor 374-8
ISSN 0002-7863
Publiceringsår 2003
Publicerad vid Institutionen för fysik (GU)
Sidor 374-8
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Biomimetic Materials/*chemistry, Biosensing Techniques, Erythrocyte Membrane/chemistry/metabolism, Humans, Liposomes/*chemistry, Membrane Proteins/*chemistry/metabolism, Microscopy, Fluorescence, Nanotechnology/*methods, Organelles/chemistry/metabolism, Signal Transduction
Ämneskategorier Kemi

Sammanfattning

We describe nanotube-vesicle networks with reconstituted membrane protein from cells and with interior activity defined by an injection of microparticles or molecular probes. The functionality of a membrane protein after reconstitution was verified by single-channel ion conductance measurements in excised inside-out patches from the vesicle membranes. The distribution of protein, determined by fluorescence detection, in the network membrane was homogeneous and could diffuse via a nanotube connecting two vesicles. We also show how injecting small unilamellar protein-containing vesicles can differentiate the contents of individual containers in a network. The combination of membrane activity and interior activity was demonstrated by ionophore-assisted accumulation, and internal Calcium Green-mediated detection, of Ca2+ within a single network container. This system can model a variety of biological functions and complex biological multicompartment structures and might serve as a platform for constructing complex sensor and computational devices.

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