Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Parallel analysis of muco… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Parallel analysis of mucosally derived B- and T-cell responses to an oral typhoid vaccine using simplified methods.

Artikel i vetenskaplig tidskrift
Författare Anna Lundgren
Joanna Kaim
Marianne Jertborn
Publicerad i Vaccine
Volym 27
Nummer/häfte 33
Sidor 4529-36
ISSN 1873-2518
Publiceringsår 2009
Publicerad vid Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi
Sidor 4529-36
Språk en
Länkar dx.doi.org/10.1016/j.vaccine.2009.0...
Ämnesord Administration, Oral, Adult, Antibodies, Bacterial, blood, immunology, Antigens, Bacterial, immunology, B-Lymphocytes, immunology, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Female, Granzymes, immunology, Humans, Immunity, Mucosal, Interferon-gamma, metabolism, Leukocytes, Mononuclear, immunology, Lymphocyte Activation, Male, T-Lymphocytes, immunology, Typhoid Fever, immunology, prevention & control, Typhoid-Paratyphoid Vaccines, immunology, Young Adult
Ämneskategorier Mikrobiologi inom det medicinska området

Sammanfattning

There is a great need for simple methods for analysis of immune responses to mucosal vaccines that can be used on small blood volumes in field trials in both children and adults. We have investigated if mucosally derived B- and T-cell responses can be monitored in parallel by analysis of antibodies and T-cell effector molecules in culture supernatants from circulating blood lymphocytes obtained from orally vaccinated Swedes. Immunization with a live oral model vaccine, i.e. Salmonellaenterica serovar Typhi Ty21a, gave rise to secretion of typhoid specific IgA and IgM antibodies from peripheral blood mononuclear cells (PBMCs) and this response could be equally well detected by ELISA and ELISPOT 7 days after vaccination. The ELISA based assay could be further simplified by using buffy coat cells, but not by using whole blood or frozen PBMCs. Vaccine induced T-cell responses appeared later than the B-cell responses, but could be detected by ELISA assessment of IFN-gamma and granzymes in supernatants from antigen stimulated PBMCs 21 days after vaccination. Thus, both B- and T-cell responses could be detected using simple ELISA based assays that would be practical to use in large-scale vaccine trials.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?