Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Tumor cell MUC1 and CD43 … - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Tumor cell MUC1 and CD43 are glycosylated differently with sialyl-Lewis a and x epitopes and show variable interactions with E-selectin under physiological flow conditions.

Artikel i vetenskaplig tidskrift
Författare Julia Fernandez-Rodriguez
O Dwir
R Alon
Gunnar C. Hansson
Publicerad i Glycoconjugate journal
Volym 18
Nummer/häfte 11-12
Sidor 925-30
ISSN 0282-0080
Publiceringsår 2001
Publicerad vid Institutionen för medicinsk och fysiologisk kemi
Sidor 925-30
Språk en
Länkar www.ncbi.nlm.nih.gov/entrez/query.f...
Ämnesord Adenocarcinoma, immunology, metabolism, Animals, Antigens, CD, Antigens, CD43, CHO Cells, metabolism, Carbohydrate Sequence, Cell Adhesion, physiology, Colonic Neoplasms, immunology, metabolism, Cricetinae, E-Selectin, genetics, metabolism, Epitopes, metabolism, Gangliosides, immunology, Glycosylation, HL-60 Cells, pathology, Humans, Molecular Sequence Data, Mucin-1, immunology, metabolism, Oligosaccharides, immunology, Rabbits, Sialoglycoproteins, immunology, metabolism, Stress, Mechanical, Tumor Cells, Cultured
Ämneskategorier Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

Sammanfattning

The mucins secreted from the colon carcinoma cell line COLO 205 have the MUC1 and CD43 (leukosialin) as core proteins, where both carry sialyl-Lewis a and MUC1 sialyl-Lewis x epitopes. The adhesion of E-selectin expressing CHO cells to the coated mucins was analyzed in a flow system revealing that the MUC1 mucin adhered better than the CD43 mucin. One reason could be their different glycosylation, a difference that was explored by analyzing the biosynthesis of MUC1 and CD43 in COLO 205 cells. Both the MUC1 and CD43 mucins became sialyl-Lewis a reactive, but after different times as revealed by pulse-chase studies. However, only MUC1 became sialyl-Lewis x reactive. These differences suggest that MUC1 and CD43 are synthesized in different compartments of the cell. It was also observed that the mucins from colon carcinoma patients had MUC1-type mucins that carried both sialyl-Lewis a and x epitopes and CD43-type sialyl-Lewis a mucins with only low levels of sialyl-Lewis x epitopes. One could hypothesize that colon carcinoma derived MUC1 is decorated with potent E-selectin epitopes, and that this could be one of several reasons for the involvement of MUC1 in cancer development.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?