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Immunogenic and adjuvant properties of Haemophilus ducreyi lipooligosaccharides.

Artikel i vetenskaplig tidskrift
Författare Annika Lundqvist
Joanna Kubler-Kielb
Susann Teneberg
Karin Ahlman
Teresa Lagergård
Publicerad i Microbes and infection / Institut Pasteur
Volym 11
Nummer/häfte 3
Sidor 352-60
ISSN 1769-714X
Publiceringsår 2009
Publicerad vid Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi
Sidor 352-60
Språk en
Länkar dx.doi.org/10.1016/j.micinf.2008.12...
Ämnesord Adjuvants, Immunologic, administration & dosage, isolation & purification, pharmacology, Animals, Antibodies, Bacterial, blood, Cells, Cultured, Haemophilus ducreyi, chemistry, immunology, Humans, Immunoglobulin G, blood, Injections, Subcutaneous, Leukocytes, Mononuclear, immunology, Lipopolysaccharides, administration & dosage, immunology, isolation & purification, pharmacology, Mice, Mice, Inbred BALB C, Mice, Knockout, Myeloid Differentiation Factor 88, immunology, Serum Albumin, Bovine, immunology, Toll-Like Receptor 4, immunology, Tumor Necrosis Factor-alpha, secretion
Ämneskategorier Mikrobiologi inom det medicinska området, Kemi

Sammanfattning

Haemophilus ducreyi, the chancroid-causing bacterium, produces lipooligosaccharides (HdLOS) that comprise 5-11 partially sialylated monosaccharides. Subcutaneous immunisation of mice with 5 microg of HdLOS purified from H. ducreyi strains 4438 and 7470 induced high levels of anti-HdLOS IgG. The antibody responses displayed T-cell-independent features, and were dependent upon Toll-like receptor 4/MyD88 signalling pathways as demonstrated using knockout mice. The immunogenicity of HdLOS was found to require the intact lipid A moiety. The specificity studies of the anti-HdLOS antibodies, as revealed by absorption studies, antibody detection in ELISA, and immune thin-layer chromatography, indicated that the majority of the anti-LOS antibodies were specific for the inner core of the HdLOS. Antibodies to HdLOS failed to inhibit LOS induction of TNF-alpha release from human mononuclear cells. The adjuvanticity of HdLOS7470 was assessed in BALB/c mice that were immunised with bovine serum albumin (BSA) with or without the addition of HdLOS. The addition of 5 microg HdLOS resulted in a 10-fold increase in the total anti-BSA IgG antibody level as estimated by ELISA. The highest increase was noted for IgG2b, which contrasted with the predominantly IgG1 subclass response to immunisation with BSA alone, indicating an immunomodulatory activity of the HdLOS.

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