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Proteomic studies in zebrafish liver cells exposed to the brominated flame retardants HBCD and TBBPA.

Artikel i vetenskaplig tidskrift
Författare Peter Kling
Lars Förlin
Publicerad i Ecotoxicology and environmental safety
Volym 72
Nummer/häfte 7
Sidor 1985-93
ISSN 1090-2414
Publiceringsår 2009
Publicerad vid Zoologiska institutionen, zoofysiologi
Sidor 1985-93
Språk en
Länkar dx.doi.org/10.1016/j.ecoenv.2009.04...
Ämnesord Animals, Biological Markers, analysis, metabolism, Cell Culture Techniques, Cell Survival, drug effects, Cells, Cultured, Electrophoresis, Gel, Two-Dimensional, Environmental Monitoring, Flame Retardants, toxicity, Fourier Analysis, Hydrocarbons, Brominated, toxicity, Liver, cytology, drug effects, metabolism, Polybrominated Biphenyls, toxicity, Proteome, metabolism, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Water Pollutants, Chemical, toxicity, Zebrafish, growth & development, metabolism
Ämneskategorier Biologiska vetenskaper

Sammanfattning

Proteomic effect screening in zebrafish liver cells was performed to generate hypotheses regarding single and mixed exposure to the BFRs HBCD and TBBPA. Responses at sublethal exposure were analysed by two-dimensional gel electrophoresis followed by MALDI-TOF and FT-ICR protein identification. Mixing of HBCD and TBBPA at sublethal doses of individual substances seemed to increase toxicity. Proteomic analyses revealed distinct exposure-specific and overlapping responses suggesting novel mechanisms with regard to HBCD and TBBPA exposure. While distinct HBCD responses were related to decreased protein metabolism, TBBPA revealed effects related to protein folding and NADPH production. Overlapping responses suggest increased gluconeogenesis (GAPDH and aldolase) while distinct mixture effects suggest a pronounced NADPH production and changes in proteins related to cell cycle control (prohibitin and crk-like oncogene). We conclude that mixtures containing HBCD and TBBPA may result in unexpected effects highlighting proteomics as a sensitive tool for detecting and hypothesis generation of mixture effects.

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