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Identification of complement C3 as an autoantigen in inflammatory bowel disease.

Artikel i vetenskaplig tidskrift
Författare Lundgren
Rorsman
Portela-Gomes
Grimelius
Ekdahl
Nilsson
Olov Ekwall
Publicerad i European journal of gastroenterology & hepatology
Volym 22
Nummer/häfte 4
Sidor 429-436
ISSN 1473-5687
Publiceringsår 2010
Publicerad vid Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning
Sidor 429-436
Språk en
Länkar dx.doi.org/10.1097/MEG.0b013e328332...
Ämnesord utoantibody, complement C3, Crohn's disease, goblet cell, inflammatory bowel disease, mucin, primary sclerosing cholangitis, ulcerative colitis
Ämneskategorier Klinisk immunologi, Gastroenterologi

Sammanfattning

OBJECTIVE: Autoantibodies against goblet cells in the gastrointestinal mucosa have been described in patients with inflammatory bowel disease (IBD) but a corresponding autoantigen has not yet been identified. The aim of this study was to identify such an antigen. METHODS: First, 10 candidate autoantigens were discarded based on double stainings of appendiceal sections and a mucin-producing cell line (HT29-mtx). Second, an appendiceal cDNA library was immunoscreened with IBD sera. RESULTS: Three out of 48 positive clones were identified as complement C3. Using immunoprecipitation of in vitro transcribed and translated C3, seven of 17 primary sclerosing cholangitis patient sera, 15 of 65 IBD sera, and none out of 54 sera from healthy blood donors showed C3 immunoreactivity. The results were confirmed using western blot and an enzyme-linked immunosorbent assay with alternative sources of C3 protein. CONCLUSION: In conclusion, we have identified complement C3 as a potential autoantigen in IBD and primary sclerosing cholangitis.

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