Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

Adenovirus 5 vector genet… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

Adenovirus 5 vector genetically re-targeted by an Affibody molecule with specificity for tumor antigen HER2/neu.

Artikel i vetenskaplig tidskrift
Författare Maria K Magnusson
Petra Henning
Susanna Myhre
M Wikman
T G Uil
M Friedman
S S Hong
R C Hoeben
N A Habib
S Ståhl
P Boulanger
Leif Lindholm
Karin Andersson
Publicerad i Cancer gene therapy
Volym 14
Nummer/häfte 5
Sidor 468-79
ISSN 0929-1903
Publiceringsår 2007
Publicerad vid Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi
Sidor 468-79
Språk en
Länkar dx.doi.org/10.1038/sj.cgt.7701027
Ämnesord Adenoviridae, genetics, Antigens, Neoplasm, genetics, immunology, Breast Neoplasms, therapy, Female, Gene Therapy, methods, Genetic Vectors, genetics, Humans, Ligands, Ovarian Neoplasms, therapy, Receptor, erbB-2, genetics, immunology, Recombinant Fusion Proteins, genetics, immunology, Tumor Cells, Cultured
Ämneskategorier Mikrobiologi inom det medicinska området

Sammanfattning

In order to use adenovirus (Ad) type 5 (Ad5) for cancer gene therapy, Ad needs to be de-targeted from its native receptors and re-targeted to a tumor antigen. A limiting factor for this has been to find a ligand that (i) binds a relevant target, (ii) is able to fold correctly in the reducing environment of the cytoplasm and (iii) when incorporated at an optimal position on the virion results in a virus with a low physical particle to plaque-forming units ratio to diminish the viral load to be administered to a future patient. Here, we present a solution to these problems by producing a genetically re-targeted Ad with a tandem repeat of the HER2/neu reactive Affibody molecule (ZH) in the HI-loop of a Coxsackie B virus and Ad receptor (CAR) binding ablated fiber genetically modified to contain sequences for flexible linkers between the ZH and the knob sequences. ZH is an Affibody molecule specific for the extracellular domain of human epidermal growth factor receptor 2 (HER2/neu) that is overexpressed in inter alia breast and ovarian carcinomas. The virus presented here exhibits near wild-type growth characteristics, infects cells via HER2/neu instead of CAR and represents an important step toward the development of genetically re-targeted adenoviruses with clinical relevance.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?