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Proteomics and peptidomics in neuroscience. Experience of capabilities and limitations in a neurochemical laboratory.

Forskningsöversiktsartikel
Författare Linda Paulson
Rita Persson
Gösta Karlsson
Jerzy Silberring
Anna Bierczynska-Krzysik
Rolf Ekman
Ann Brinkmalm-Westman
Publicerad i Journal of mass spectrometry : JMS
Volym 40
Nummer/häfte 2
Sidor 202-13
ISSN 1076-5174
Publiceringsår 2005
Publicerad vid Institutionen för klinisk neurovetenskap
Sidor 202-13
Språk en
Länkar dx.doi.org/10.1002/jms.740
Ämnesord Animals, Brain Chemistry, Cells, Cultured, Disease Models, Animal, Laboratories, Hospital, Mental Disorders, diagnosis, therapy, Neurochemistry, methods, Neurodegenerative Diseases, diagnosis, prevention & control, Neurosciences, Proteomics, methods, Specimen Handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Ämneskategorier Neurobiologi

Sammanfattning

The increasing use of proteomics has created a basis for new strategies to develop methodologies for rapid identification of protein patterns in living organisms. It has also become evident that proteomics has other potential applications than protein and peptide identification, e.g. protein characterization, with the aim of revealing their structure, function(s) and interactions of proteins. In comparative proteomics studies, the protein expression of a certain biological system is compared with another system or the same system under perturbed conditions. Global identification of proteins in neuroscience is extremely complex, owing to the limited availability of biological material and very low concentrations of the molecules. Moreover, in addition to proteins, there are number of peptides that must also be considered in global studies on the central nervous system. In this overview, we focus on and discuss problems related to the different sources of biological material and sample handling, which are part of all preparatory and analytical steps. Straightforward protocols are desirable to avoid excessive purification steps, since loss of material at each step is inevitable. We would like to merge the two worlds of proteomics/peptidomics and neuroscience, and finally we consider different practical and technical aspects, illustrated with examples from our laboratory.

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