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Clonal populations of chondrocytes with progenitor properties identified within human articular cartilage.

Artikel i vetenskaplig tidskrift
Författare Maria Thornemo
Tommi Tallheden
Eva Sjögren-Jansson
A Larsson
Karina Lövstedt
Ulf Nannmark
Mats Brittberg
Anders Lindahl
Publicerad i Cells, tissues, organs
Volym 180
Nummer/häfte 3
Sidor 141-50
ISSN 1422-6405
Publiceringsår 2005
Publicerad vid Institutionen för de kirurgiska disciplinerna, Avdelningen för ortopedi
Institutionen för anatomi och cellbiologi
Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin
Sidor 141-50
Språk en
Länkar dx.doi.org/10.1159/000088242
Ämnesord Adolescent, Adult, Biological Markers, Cartilage, Articular, cytology, Cell Culture Techniques, Cell Differentiation, physiology, Cell Proliferation, Cell Shape, Cells, Cultured, Chondrocytes, physiology, ultrastructure, Extracellular Matrix, chemistry, metabolism, Gene Expression, Humans, Immunohistochemistry, Middle Aged, Phenotype, Stem Cells, physiology, ultrastructure
Ämneskategorier Ortopedi

Sammanfattning

The aim of the present study was to identify and characterize progenitor properties of human articular chondrocytes selected by using agarose suspension culture. In this chondrogenic selective culture condition, about 3.6% of seeded surplus chondrocytes from patients undergoing articular chondrocyte transplantation proliferated and formed cell clusters after 6 weeks. Phase-contrast microscopy and transmission electron microscopy revealed four different types of cell clusters differing in cellular content and matrix production. Based on their morphological features, they were named the homogenous (H), the homogenous matrix (HM), the differentiated matrix (DM) and the differentiated (D) cell clusters. All cell clusters showed positive safranin O staining, and matrix was positive for antibodies detecting type II collagen and aggrecan. The clusters were further demonstrated to express the genes for fibroblast growth factor receptor 3, type IIA collagen and type IIB collagen, while type X collagen was not expressed. After subcloning, the H and HM clusters demonstrated the best proliferative capacity. Chondrocytes from these two cell clusters also showed phenotypic plasticity in chondrogenic, adipogenic as well as osteogenic assays. This study demonstrates that existing subpopulations of cells with chondroprogenitor properties can be isolated from human adult articular cartilage using agarose suspension cultures.

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