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Proliferation and differentiation potential of chondrocytes from osteoarthritic patients.

Artikel i vetenskaplig tidskrift
Författare Tommi Tallheden
Catherine Bengtsson
Camilla Brantsing
Eva Sjögren-Jansson
Lars Carlsson
Lars Peterson
Mats Brittberg
Anders Lindahl
Publicerad i Arthritis research & therapy
Volym 7
Nummer/häfte 3
Sidor R560-8
ISSN 1478-6362
Publiceringsår 2005
Publicerad vid Institutionen för de kirurgiska disciplinerna, Avdelningen för ortopedi
Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin
Sidor R560-8
Språk en
Länkar dx.doi.org/10.1186/ar1709
Ämnesord Aged, Aged, 80 and over, Cell Differentiation, physiology, Cell Proliferation, Cells, Cultured, Chondrocytes, metabolism, pathology, secretion, Female, Humans, Male, Middle Aged, Osteoarthritis, metabolism, pathology
Ämneskategorier Ortopedi

Sammanfattning

Autologous chondrocyte transplantation (ACT) has been shown, in long-term follow-up studies, to be a promising treatment for the repair of isolated cartilage lesions. The method is based on an implantation of in vitro expanded chondrocytes originating from a small cartilage biopsy harvested from a non-weight-bearing area within the joint. In patients with osteoarthritis (OA), there is a need for the resurfacing of large areas, which could potentially be made by using a scaffold in combination with culture-expanded cells. As a first step towards a cell-based therapy for OA, we therefore investigated the expansion and redifferentiation potential in vitro of chondrocytes isolated from patients undergoing total knee replacement. The results demonstrate that OA chondrocytes have a good proliferation potential and are able to redifferentiate in a three-dimensional pellet model. During the redifferentiation, the OA cells expressed increasing amounts of DNA and proteoglycans, and at day 14 the cells from all donors contained type II collagen-rich matrix. The accumulation of proteoglycans was in comparable amounts to those from ACT donors, whereas total collagen was significantly lower in all of the redifferentiated OA chondrocytes. When the OA chondrocytes were loaded into a scaffold based on hyaluronic acid, they bound to the scaffold and produced cartilage-specific matrix proteins. Thus, autologous chondrocytes are a potential source for the biological treatment of OA patients but the limited collagen synthesis of the OA chondrocytes needs to be further explained.

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