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Mid-trimester amniotic fluid proteome's association with spontaneous preterm delivery and gestational duration.

Artikel i vetenskaplig tidskrift
Författare Maria Hallingström
Petra Zedníková
Vojtěch Tambor
Malin Barman
Marie Vajrychová
Juraj Lenčo
Felicia Viklund
Linda Tancred
Hardis Rabe
Daniel Jonsson
Alisa Kachikis
Staffan Nilsson
Marian Kacerovský
Kristina M. Adams Waldorf
Bo Jacobsson
Publicerad i PloS one
Volym 15
Nummer/häfte 5
Sidor e0232553
ISSN 1932-6203
Publiceringsår 2020
Publicerad vid Institutionen för biomedicin
Institutionen för biomedicin, avdelningen för infektionssjukdomar
Institutionen för kliniska vetenskaper, Avdelningen för obstetrik och gynekologi
Sidor e0232553
Språk en
Länkar dx.doi.org/10.1371/journal.pone.023...
www.ncbi.nlm.nih.gov/entrez/query.f...
Ämneskategorier Obstetrik och kvinnosjukdomar

Sammanfattning

Amniotic fluid is clinically accessible via amniocentesis and its protein composition may correspond to birth timing. Early changes in the amniotic fluid proteome could therefore be associated with the subsequent development of spontaneous preterm delivery.The main objective of this study was to perform unbiased proteomics analysis of the association between mid-trimester amniotic fluid proteome and spontaneous preterm delivery and gestational duration, respectively. A secondary objective was to validate and replicate the findings by enzyme-linked immunosorbent assay using a second independent cohort.Women undergoing a mid-trimester genetic amniocentesis at Sahlgrenska University Hospital/Östra between September 2008 and September 2011 were enrolled in this study, designed in three analytical stages; 1) an unbiased proteomic discovery phase using LC-MS analysis of 22 women with subsequent spontaneous preterm delivery (cases) and 37 women who delivered at term (controls), 2) a validation phase of proteins of interest identified in stage 1, and 3) a replication phase of the proteins that passed validation using a second independent cohort consisting of 20 cases and 40 matched controls.Nine proteins were nominally significantly associated with both spontaneous preterm delivery and gestational duration, after adjustment for gestational age at sampling, but none of the proteins were significant after correction for multiple testing. Several of these proteins have previously been described as being associated with spontaneous PTD etiology and six of them were thus validated using enzyme linked immunosorbent assay. Two of the proteins passed validation; Neutrophil gelatinase-associated lipocalin and plasminogen activator inhibitor 1, but the results could not be replicated in a second cohort.Neutrophil gelatinase-associated lipocalin and Plasminogen activator inhibitor 1 are potential biomarkers of spontaneous preterm delivery and gestational duration but the findings could not be replicated. The negative findings are supported by the fact that none of the nine proteins from the exploratory phase were significant after correction for multiple testing.

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