Till sidans topp

Sidansvarig: Webbredaktion
Sidan uppdaterades: 2012-09-11 15:12

Tipsa en vän
Utskriftsversion

The basis for non-canonic… - Göteborgs universitet Till startsida
Webbkarta
Till innehåll Läs mer om hur kakor används på gu.se

The basis for non-canonical ROK family function in the N-acetylmannosamine kinase from the pathogen Staphylococcus aureus

Artikel i vetenskaplig tidskrift
Författare D. Coombes
J. S. Davies
M. C. Newton-Vesty
C. R. Horne
T. G. Setty
R. Subramanian
J. W. B. Moir
Rosmarie Friemann
S. Panjikar
M. D. W. Griffin
R. A. North
R. C. J. Dobson
Publicerad i Journal of Biological Chemistry
Volym 295
Nummer/häfte 10
Sidor 3301-3315
ISSN 0021-9258
Publiceringsår 2020
Publicerad vid CARe - Centrum för antibiotikaresistensforskning
Sidor 3301-3315
Språk en
Länkar dx.doi.org/10.1074/jbc.RA119.010526
Ämnesord Staphylococcus aureus (S, aureus), X-ray crystallography, analytical, ultracentrifugation, small-angle X-ray scattering (SAXS), structural, biology, N-aceytlmannosamine kinase, repressor, open reading frame, kinase, ROK protein family, sialic acid, sugar kinase, x-ray diffraction, sialic-acid catabolism, molecular replacement, escherichia-coli, acetylneuraminate lyase, crystal-structures, bacillus-subtilis, glucose kinase, purification, crystallization, Biochemistry & Molecular Biology
Ämneskategorier Biokemi

Sammanfattning

In environments where glucose is limited, some pathogenic bacteria metabolize host-derived sialic acid as a nutrient source. N-Acetylmannosamine kinase (NanK) is the second enzyme of the bacterial sialic acid import and degradation pathway and adds phosphate to N-acetylmannosamine using ATP to prime the molecule for future pathway reactions. Sequence alignments reveal that Gram-positive NanK enzymes belong to the Repressor, ORF, Kinase (ROK) family, but many lack the canonical Zn-binding motif expected for this function, and the sugar-binding EXGH motif is altered to EXGY. As a result, it is unclear how they perform this important reaction. Here, we study the Staphylococcus aureus NanK (SaNanK), which is the first characterization of a Gram-positive NanK. We report the kinetic activity of SaNanK along with the ligand?free, N-acetylmannosamine?bound and substrate analog GlcNAc?bound crystal structures (2.33, 2.20, and 2.20 ? resolution, respectively). These demonstrate, in combination with small-angle X-ray scattering, that SaNanK is a dimer that adopts a closed conformation upon substrate binding. Analysis of the EXGY motif reveals that the tyrosine binds to the N-acetyl group to select for the ?boat? conformation of N-acetylmannosamine. Moreover, SaNanK has a stacked arginine pair coordinated by negative residues critical for thermal stability and catalysis. These combined elements serve to constrain the active site and orient the substrate in lieu of Zn binding, representing a significant departure from canonical NanK binding. This characterization provides insight into differences in the ROK family and highlights a novel area for antimicrobial discovery to fight Gram-positive and S. aureus infections.

Sidansvarig: Webbredaktion|Sidan uppdaterades: 2012-09-11
Dela:

På Göteborgs universitet använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor.  Vad är kakor?